PXD059835

PXD059835 is an original dataset announced via ProteomeXchange.

Title	LC-M/MS Protein aggregation isolation is murine melanoma lines after OSGEP depletion
Description	The immune response in cancer, particularly in melanoma, is often hindered by tumour-induced immune evasion strategies, but the precise mechanisms for this remains unclear. Here, we reveal how the tRNA modification threonine-6-adenosine (t6A), mediated by O-sialoglycoprotein endopeptidase (OSGEP), maintains proteostasis in melanoma cells to prevent anti-tumour immune recognition. Cytoplasmic translation depends on the formation of t6A modification in position 37 of tRNANNU for efficient decoding of ANN codons. Defective protein homeostasis resulting from knockdown of OSGEP triggers accumulation of protein aggregates and the formation of stress granules. This disrupted codon-specific translation induces the activation of RIG-I and type I interferon response. Hence, t6A-deficient tumours prompt recruitment and activation of T-cells, affecting tumour growth. Finally, an OSGEP-driven gene signature in melanoma patients is predictive for T-cell infiltration and overall survival. Together, our results reveal t6A tRNA modification as a novel therapeutic target to fight melanoma.
HostingRepository	PRIDE
AnnounceDate	2026-04-06
AnnouncementXML	Submission_2026-04-05_16:17:24.764.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD059835
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dominique Baiwir
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-15 13:30:32	ID requested
⏵ 1	2026-04-05 16:17:25	announced

10.1038/s41467-026-69964-2;

10.6019/PXD059835;

Dziagwa C, Seca C, Capron C, Maurizy C, An N, Heusdens D, Budden T, Martin-Morales L, Susaeta Ruiz M, Renaude E, El-Hachem N, Vanleyssem R, Leclercq M, Blomme A, Chariot A, Utikal J, Viros A, Rapino F, Delaunay S, Close P, Disruption of tRNA threonylation triggers RIG-I mediated anti-tumour immune response. Nat Commun, 17(1):(2026) [pubmed]

submitter keyword: tRNA modification,Melanoma, protein misfolding

Pierre Close
contact affiliation	Lab of Cancer Signaling, GIGA-Institute, ULiege, Belgium
contact email	pierre.close@uliege.be
lab head
Dominique Baiwir
contact affiliation	University of Liege
contact email	d.baiwir@ulg.ac.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD059835

PRIDE project URI

• PRIDE
  1. PXD059835
     1. Label: PRIDE project
     2. Name: LC-M/MS Protein aggregation isolation is murine melanoma lines after OSGEP depletion