PXD059829

PXD059829 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteomic and Phosphoproteomic Sequencing of NRK-49F Cells after TGF-β1 Stimulation: Impact of USP7 Knockout
Description	Dysregulation of the expression of ubiquitin - specific protease 7 (USP7) has been associated with tumor progression. However, the biological functions and clinical significance of USP7 in renal fibrosis remain insufficiently explored. In this study, proteomic and phosphoproteomic sequencing were performed on NRK - 49F cells with and without USP7 knockout after TGF - β1 stimulation. The aim was to explore the possible mechanism of action of USP7 in the fibrosis process of renal fibroblasts, so as to confirm that USP7 is a potential therapeutic target for alleviating renal fibrosis, and that inhibiting USP7 may be an effective strategy for treating chronic kidney disease (CKD).
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-06_17:00:16.313.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shiqi Lv
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116;
ModificationList	phosphorylated residue
Instrument	LCQ Classic

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-15 10:35:42	ID requested
⏵ 1	2026-09-06 17:00:17	announced

Publication List

Lv S, Shen Z, Gu Y, Wang Y, Wang J, Zhang H, Liu H, Chen J, Zhu C, Huang X, Liu T, Ding X, Zhang X, Deubiquitinase USP7 stabilizes the histone demethylase KDM5B and promotes the progression of renal fibrosis through the TSC1/mTOR axis. Mol Biomed, 7(1):(2026) [pubmed]
10.1186/s43556-026-00531-3;

Lv S, Shen Z, Gu Y, Wang Y, Wang J, Zhang H, Liu H, Chen J, Zhu C, Huang X, Liu T, Ding X, Zhang X, Deubiquitinase USP7 stabilizes the histone demethylase KDM5B and promotes the progression of renal fibrosis through the TSC1/mTOR axis. Mol Biomed, 7(1):(2026) [pubmed]

10.1186/s43556-026-00531-3;

Keyword List

submitter keyword: NRK-49F;USP7

submitter keyword: NRK-49F;USP7

Contact List

Xiaoyan Zhang
contact affiliation	Kidney and Dialysis Institute of Shanghai
contact email	zs_xyzhang@163.com
lab head
Shiqi Lv
contact affiliation	Fudan university
contact email	13301050097@fudan.edu.cn
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD059829

PRIDE project URI

Repository Record List

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