PXD059820

PXD059820 is an original dataset announced via ProteomeXchange.

Title	Feeder-free culture of naive human PSCs preserving embryonic, extraembryonic and blastoid potential
Description	Naive human pluripotent stem cells (hPSCs) represent a pre-implantation epiblast state able to efficiently differentiate into embryonic and extraembryonic pre-implantation lineages and to self-organise into blastocyst-like structures called blastoids. Naive hPSCs maintenance commonly relies on mouse embryonic fibroblast (MEFs), introducing variability and analytical confounders. Here, we describe a feeder-free culture system based on serum coating that supports long-term naive hPSC maintenance. Across five laboratories, 30 serum batches were evaluated for the expansion of 8 naive hPSC lines for up to 25 passages. Mass spectrometry identified fibronectin and collagens as extracellular matrix proteins consistently present in serum coating. Cells cultured on serum coating displayed growth kinetics, clonogenic capacity, mutation rates, and global gene expression profiles comparable to MEF-based cultures. Importantly, serum-cultured naive hPSCs efficiently underwent germ layer specification, retained trophectoderm competence, and generated blastoids with similar efficiency. Collectively, serum coating provides a scalable, cost-effective, and robust alternative to feeder-based systems, preserving genomic stability and developmental potential while eliminating MEF-associated disadvantages and variability. This platform facilitates large-scale applications of naive hPSCs and enables more reproducible mechanistic studies.
HostingRepository	PRIDE
AnnounceDate	2025-12-22
AnnouncementXML	Submission_2025-12-22_08:51:41.427.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Graziano Martello
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116; scientific name: Bos taurus (Bovine); NCBI TaxID: NEWT:9913;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-15 06:32:08	ID requested
⏵ 1	2025-12-22 08:51:41	announced

Dataset with its publication pending

submitter keyword: None

Graziano Martello
contact affiliation	University of Padua
contact email	graziano.martello@unipd.it
lab head
Graziano Martello
contact affiliation	Department of Molecular Medicine, Medical School, University of Padua, Padua 35121, Italy.
contact email	graziano.martello@unipd.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD059820

PRIDE project URI

• PRIDE
  1. PXD059820
     1. Label: PRIDE project
     2. Name: Feeder-free culture of naive human PSCs preserving embryonic, extraembryonic and blastoid potential