PXD059813

PXD059813 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of the exoproteome and surfaceome of Lactiplantibacillus plantarum CRL681: mechanisms of competitive exclusion against enterohemorrhagic Escherichia coli
Description	The study focuses on the proteomic analysis of the exoproteome and surfaceome of Lactiplantibacillus plantarum CRL681, aiming to explore the molecular mechanisms underlying its antagonistic capacity against enterohemorrhagic Escherichia coli (EHEC). More than 600 proteins were identified by mass spectrometry across both proteomes, of which 285 were characterized as part of the secretome through a detailed analysis with bioinformatics tools and bibliographic references. The analysis of these proteins allowed us to conclude that some of them are involved in functions like energy metabolism, nutrient transport, cell adhesion, genetic information processing, and signaling. Additionally, moonlighting proteins were observed, which possess bacterial adhesion capabilities that may contribute to the L. plantarum CRL681 antagonistic actions against EHEC. This group included ribosomal proteins and peptidoglycan hydrolases, that would be responsible for the EHEC cell lysis observed under in vitro experimental conditions. Furthermore, the co-culture of the CRL681 strain with E. coli revealed differential expression of key proteins, such as the overexpression of glutathione reductase, involved in maintaining redox balance. This proteomic study is a step forward in the understanding of the molecular mechanisms underlying the antagonistic capacity of L. plantarum CRL681 against EHEC.
HostingRepository	PRIDE
AnnounceDate	2026-02-02
AnnouncementXML	Submission_2026-02-01_16:33:33.195.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Leonardo Albarracin
SpeciesList	scientific name: Lactobacillus plantarum; NCBI TaxID: NEWT:1590; scientific name: Escherichia coli; NCBI TaxID: NEWT:562; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-15 04:37:28	ID requested
⏵ 1	2026-02-01 16:33:34	announced

Baillo AA, Albarrac, í, n L, Heredia Ojeda E, Elean M, Gong W, Kitazawa H, Villena J, Fadda S, O157:H7. Antibiotics (Basel), 15(1):(2026) [pubmed]

10.3390/antibiotics15010096;

submitter keyword: surfaceoma, Exoprotome, enterohemorrhagic Escherichia coli,Lactiplantibacillus plantarum

Julio Villena
contact affiliation	Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET)
contact email	jcvillena@cerela.org.ar
lab head
Leonardo Albarracin
contact affiliation	CERELA - CONICET
contact email	lalbarracin@herrera.unt.edu.ar
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD059813

PRIDE project URI

• PRIDE
  1. PXD059813
     1. Label: PRIDE project
     2. Name: Proteomic analysis of the exoproteome and surfaceome of Lactiplantibacillus plantarum CRL681: mechanisms of competitive exclusion against enterohemorrhagic Escherichia coli