PXD059742

PXD059742 is an original dataset announced via ProteomeXchange.

Title	Regulation of circulating mannosylated proteome by macrophage mannose receptor, Mrc1
Description	Abundance of secreted proteins is tightly regulated in circulation, and its dysregulation forms a basis for development of pathology. N-glycosylation has been shown to be a decisive factor in determining the half-life of these proteins. Exoglycosidases remodel the proteins and exposure of cryptic ligands of endocytic lectin receptors determine their half-lives. We have devised a strategy for non-denaturing enrichment of these mannosylated proteins upon Mrc1 lectin receptor deficiency. Data-independent acquisition mass spectrometry was used to quantify these proteins post-enrichment by mannose-binding lectin (Concanavalin A). We find ligand profile of Mrc1 comprises of over 200 proteins, linking inflammation, organ dysfunction and sepsis to turnover of mannosylated blood proteome. Mannosylated proteome was profiled in mouse plasma upon Mrc1 knock-out and in human plasma upon sepsis. Pathway enrichment was performed to draw parallels between Mrc1 deficiency in mice and sepsis in human.
HostingRepository	PRIDE
AnnounceDate	2025-06-11
AnnouncementXML	Submission_2025-06-11_10:22:23.608.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jamey Marth
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-13 17:14:01	ID requested
⏵ 1	2025-06-11 10:22:24	announced

Dataset with its publication pending

submitter keyword: Plasma, Mrc1, mannosylated proteins, sepsis

Jamey Marth
contact affiliation	Sanford Burnham Prebys Medical Discovery Institute
contact email	jmarth@sbpdiscovery.org
lab head
Jamey Marth
contact affiliation	Sanford Burnham Prebys Medical Discovery Institute, La Jolla
contact email	jmarth@sbpdiscovery.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD059742

PRIDE project URI

• PRIDE
  1. PXD059742
     1. Label: PRIDE project
     2. Name: Regulation of circulating mannosylated proteome by macrophage mannose receptor, Mrc1