PXD059709

PXD059709 is an original dataset announced via ProteomeXchange.

Title	Histone Lysine β-Hydroxybutyrylation Regulates Drought Response in Rice
Description	Protein post-translational modifications (PTMs), such as acetylation and methylation, are established as crucial epigenetic marks in eukaryotic cells. Recent advances in mass spectrometry have led to the discovery of novel PTMs, including crotonylation, 2-hydroxyisobutyrylation, β-hydroxybutyrylation, lactylation, and acetoacetylation. These emerging PTMs are increasingly recognized for their significant roles in diverse cellular processes, including gene transcription, protein stability, enzyme activity, and protein interactions. Lysine β-hydroxybutyrylation (Kbhb) has been well characterized in animal cells, particularly for its influence on energy metabolism. For instance, the β-hydroxybutyrylation of S-adenosyl-L-homocysteine hydrolase (AHCY), a critical enzyme in the methionine cycle, has been shown to impact metabolite levels. Kbhb modifications on histones are also known to regulate gene expression, contributing to various physiological processes, including immune responses, diabetic cardiomyopathy, and postnatal heart development . Notably, the detection of Kbhb in microbial species suggests that it may represent a conserved PTM across life forms. However, studies on Kbhb in plants remain unexplored. To address this gap, we conducted a comprehensive mass spectrometry analysis of the β-hydroxybutyrylome in rice. Our results revealed that a substantial number of rice proteins were modified by β-hydroxybutyrylation, and functional analysis demonstrated that β-hydroxybutyrylation positively regulates drought stress responses in rice.
HostingRepository	PRIDE
AnnounceDate	2026-03-09
AnnouncementXML	Submission_2026-03-08_17:35:11.424.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qiutao Xu
SpeciesList	scientific name: Oryza sativa (Rice); NCBI TaxID: NEWT:4530;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-12 22:19:47	ID requested
⏵ 1	2026-03-08 17:35:12	announced

Wei X, Chen Z, Liu Y, Xiao G, Guo S, Huang J, Ren W, Ma X, Chen X, Zhang J, Xu Q, -hydroxybutyrylation as a drought-responsive epigenetic mark in rice. Cell Discov, 12(1):(2026) [pubmed]

10.1038/s41421-026-00868-7;

submitter keyword: Rice

Histone

lysine β-hydroxybutyrylation

epigenetics

Qiutao Xu
contact affiliation	Affiliation of Qiutao Xu: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Agriculture, Guangxi University, Nanning 530004, China
contact email	qiutaoxu@gxu.edu.cn
lab head
Qiutao Xu
contact affiliation	Guangxi University
contact email	qiutaoxu@gxu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD059709
     1. Label: PRIDE project
     2. Name: Histone Lysine β-Hydroxybutyrylation Regulates Drought Response in Rice