PXD059686

PXD059686 is an original dataset announced via ProteomeXchange.

Title	Proteomics reveals that vitamin D deficiency leads to immunoglobulin abnormalities and immune dysregulation in patients with post–COVID-19 condition
Description	Background: Vitamin D (VD) has a coordinated immune function. Decreased VD levels are closely related to the occurrence of post-COVID-19 Conditions (PCC), but the specific mechanism is still unclear. Methods: We collected 50 PCC patient serum samples and divided them into three groups by different VD levels: VD sufficient group (VD ≥ 30 ng/mL), VD insufficient group (20 ng/mL ≤ VD < 30 ng/mL), VD deficiency group (VD < 20 ng/mL) and then subjected to proteomic analysis. Results: We identified 15 differential abundance proteins (DAPs) between the VD sufficient and VD insufficient groups, among which 11 DAPs were up-regulated and 4 DAPs were down-regulated in the VD insufficient group, including 5 immunoglobulin proteins (JCHAJN, IGHV4-28, GHV4-34, IGHM, and IGLV2-11), a complement component C7, and CD5L. Except for C7, the remaining 6 DAPs were significantly negatively correlated with VD levels in PCC patients. These DAPs were primarily enriched in immune-related pathways such as TNF signaling pathway and B cell receptor signaling pathway. Moreover, compared with the VD insufficient group, VD deficiency resulted in an abnormal abundance of 12 proteins, of which 4 proteins were up-regulated and 8 proteins were down-regulated, such as VD deficiency leads to a declined abundance of IGLV1-47 negatively correlated with serum VD levels. These 12 DAPs were also broadly involved in immune-related pathways. Furthermore, we found that ALB was in the center of the PPI network map of all DAPs and was negatively correlated with serum VD levels. Conclusion: VD insufficient/deficient leads to abnormalities in immunoglobulin heavy, light, and J chains, resulting in immune system dysfunction leading to PCC syndrome. VD supplementation may be a potential therapeutic strategy to alleviate the symptoms of PCC.
HostingRepository	iProX
AnnounceDate	2025-01-10
AnnouncementXML	Submission_2025-01-12_17:54:08.792.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaomin Xie
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-12 17:53:45	ID requested
⏵ 1	2025-01-12 17:54:09	announced

Dataset with its publication pending

submitter keyword: Post-COVID-19

vitamin D insufficient/deficient

immunoglobulin

Xiaomin Xie
contact affiliation	The First People's Hospital of Yinchuan
contact email	xxm2324@126.com
lab head
Xiaomin Xie
contact affiliation	The First People's Hospital of Yinchuan
contact email	xxm2324@126.com
dataset submitter

iProX dataset URI