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PXD059670

PXD059670 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCombinatorial phosphorylation on CTD of RNA polymerase II selectively controls transcription and export of protein-coding mRNAs
DescriptionC-terminal domain (CTD) of RNA polymerase II is crucial for recruiting transcription regulators via specific post-translational modifications (PTM), especially phosphorylation. The hypothesis of combination of PTMs, or CTD code, that can allow precise and dynamic recruitment of transcription machinery is highly attractive, yet the experimental evidence to support this hypothesis has been scarce. Here, despite lacking specific antibodies for combinatorial CTD phosphorylation, we developed an innovative approach that detects double phosphorylation patterns on the CTD in a whole-genomic fashion by leveraging the antibody masking effect with selectively removing the flanking interference. Using this method, we detected pT4pS5 double phosphosites occurring exclusively during the transcription of protein-coding genes. Furthermore, we showed that pT4pS5 marks recruit the Transcription and Export complex (TREX), which specifically facilitates mRNA processing and nucleocytoplasmic export of protein-coding mRNAs. The recruitment of TREX by pT4pS5 phosphosites is particularly important for the processing of lengthy neurogenesis-related genes. Our results provide experimental support for the notion that CTD coding system can function combinatorically and in a gene-specific manner, which encodes an exact information about the transcription of specific gene clusters. This method can be broadly applied to map all combinatorial PTM patterns on RNA polymerase II, paving the way for a deeper understanding of gene-specific transcription regulation at the molecular level
HostingRepositoryMassIVE
AnnounceDate2025-01-15
AnnouncementXMLSubmission_2025-01-15_14:31:28.784.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterBrendan Floyd
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListOxidation; Carbamidomethyl
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-01-11 11:32:36ID requested
12025-01-15 14:31:29announced
Publication List
no publication
Keyword List
submitter keyword: Phosphorylation, PPI, DatasetType:Proteomics
Contact List
Edward Marcotte
contact affiliationUniversity of Texas-Austin
contact emailmarcotte@icmb.utexas.edu
lab head
Brendan Floyd
contact affiliationStanford University
contact emailbmfloyd@stanford.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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