PXD059669 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic study of evolved P. aeruginosa strains grown in S. aureus- and K. pneumoniae-conditioned media |
Description | In this research, Pseudomonas aeruginosa underwent evolution in the supernatant of either S. aureus (SA) or K. pneumoniae (KP). Through this evolution process, we enriched a sufficient number of bacterial cells along with their corresponding proteins. Following 15 cultivation cycles, notable changes were observed in the phenotypes of P. aeruginosa, with a series of mutations identified through whole-genome sequencing (WGS). Proteomics analysis unveiled that the supernatant-evolved mutants displayed distinct regulation patterns in crucial pathways (including the type VI secretion system, biofilm formation, phenazine biosynthesis, translation, beta-lactam resistance, and O-antigen biosynthesis) in comparison to both the ancestral strain and the unmodified medium-evolved (UmMd-evolved) strain. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-21 |
AnnouncementXML | Submission_2025-05-20_22:02:45.383.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yanrong Pan |
SpeciesList | scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964; |
ModificationList | No PTMs are included in the dataset |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2025-01-11 08:09:10 | ID requested | |
⏵ 1 | 2025-05-20 22:02:45 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: supernatant, laboratory evolution, proteomics, K. pneumoniae, S. aureus,P. aeruginosa |
Contact List
Henry Lam |
contact affiliation | Chemical and Biological Engineering, The Hong Kong University of Science and Technology (HKUST), Hong Kong (lab head) |
contact email | kehlam@ust.hk |
lab head | |
Yanrong Pan |
contact affiliation | Hong Kong University of Science and Technology |
contact email | ypanbe@connect.ust.hk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD059669
- Label: PRIDE project
- Name: Proteomic study of evolved P. aeruginosa strains grown in S. aureus- and K. pneumoniae-conditioned media