PXD059669

PXD059669 is an original dataset announced via ProteomeXchange.

Title	Proteomic study of evolved P. aeruginosa strains grown in S. aureus- and K. pneumoniae-conditioned media
Description	In this research, Pseudomonas aeruginosa underwent evolution in the supernatant of either S. aureus (SA) or K. pneumoniae (KP). Through this evolution process, we enriched a sufficient number of bacterial cells along with their corresponding proteins. Following 15 cultivation cycles, notable changes were observed in the phenotypes of P. aeruginosa, with a series of mutations identified through whole-genome sequencing (WGS). Proteomics analysis unveiled that the supernatant-evolved mutants displayed distinct regulation patterns in crucial pathways (including the type VI secretion system, biofilm formation, phenazine biosynthesis, translation, beta-lactam resistance, and O-antigen biosynthesis) in comparison to both the ancestral strain and the unmodified medium-evolved (UmMd-evolved) strain.
HostingRepository	PRIDE
AnnounceDate	2025-05-21
AnnouncementXML	Submission_2025-05-20_22:02:45.383.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yanrong Pan
SpeciesList	scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-11 08:09:10	ID requested
⏵ 1	2025-05-20 22:02:45	announced

Dataset with its publication pending

submitter keyword: supernatant, laboratory evolution, proteomics, K. pneumoniae, S. aureus,P. aeruginosa

Henry Lam
contact affiliation	Chemical and Biological Engineering, The Hong Kong University of Science and Technology (HKUST), Hong Kong (lab head)
contact email	kehlam@ust.hk
lab head
Yanrong Pan
contact affiliation	Hong Kong University of Science and Technology
contact email	ypanbe@connect.ust.hk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD059669

PRIDE project URI

• PRIDE
  1. PXD059669
     1. Label: PRIDE project
     2. Name: Proteomic study of evolved P. aeruginosa strains grown in S. aureus- and K. pneumoniae-conditioned media