PXD059663

PXD059663 is an original dataset announced via ProteomeXchange.

Title	Ascorbate peroxidase (APEX2) -mediated proximity-based proteomics for HTT protein interactions
Description	Huntington’s Disease (HD), a progressive neurodegenerative disorder with no disease-modifying therapies, is caused by a CAG repeat expansion in the HD gene encoding polyglutamine-expanded huntingtin (HTT) protein. Mechanisms of HD cellular pathogenesis and cellular functions of the normal and mutant HTT proteins are still not completely understood. HTT protein has numerous interaction partners, and it likely provides a scaffold for assembly of multiprotein complexes many of which may be altered in HD. Previous studies have implicated DNA damage response in HD pathogenesis. Gene transcription and RNA processing has also emerged as molecular mechanisms associated with HD. Here we used multiple approaches to identify HTT interactors in the context of DNA damage stress. We present evidence for the role for HTT in double strand break (DSB) repair mechanism. Our results indicate that HTT interacts with many proteins involved in the regulation of interconnected DNA repair/remodeling and RNA processing pathways.
HostingRepository	PRIDE
AnnounceDate	2026-03-16
AnnouncementXML	Submission_2026-03-16_09:37:38.032.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD059663
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Tamara Ratovitski
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-10 15:18:08	ID requested
⏵ 1	2026-03-16 09:37:38	announced

10.6019/PXD059663;

submitter keyword: Huntington’s Disease, protein interactions, huntingtin, DNA damage, neurodegeneration

Christopher A. Ross
contact affiliation	Division of Neurobiology, Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, MD
contact email	caross@jhu.edu
lab head
Tamara Ratovitski
contact affiliation	Division of Neurobiology, Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore MD
contact email	tratovi1@jhmi.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD059663
     1. Label: PRIDE project
     2. Name: Ascorbate peroxidase (APEX2) -mediated proximity-based proteomics for HTT protein interactions