PXD059610 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Harnessing hydrodynamics for high-yield production of extracellular vesicles from stem cells spheroids with specific cargo profiling |
| Description | This study presents a novel method and device for the hydrodynamic production of extracellular vesicles (EVs) derived from biomimetic multicellular 3D spheroids, enabling high-throughput particle release that is 10 to 20 times higher than in non-stimulated conditions. The device facilitates the formation of spheroids from human mesenchymal stem cells (hMSCs), offering an all-in-one approach for both spheroid generation and EV release. Production times are reduced to just a few hours, with yield further increased by alternating periods of high hydrodynamic flow and spheroid recovery in a sequential production approach. Using this system, we explored the impact of hydrodynamic and starvation conditions on the protein cargo of EVs, identifying distinct protein markers through proteomics. Specifically, hydrodynamic stimulation enriched EVs in plasma membrane-derived and mitochondrial proteins, revealing divergent biogenesis pathways. Importantly, the produced EVs exhibited therapeutic properties, with demonstrated effects in wound healing, angiogenesis, and anti-inflammatory responses, some showing enhanced efficacy under hydrodynamic stimulation. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-19 |
| AnnouncementXML | Submission_2026-02-19_10:47:14.846.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Vanessa Masson |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-01-09 15:18:53 | ID requested | |
| ⏵ 1 | 2026-02-19 10:47:15 | announced | |
Publication List
Keyword List
| submitter keyword: Extracellular vesicles, Hydrodynamic stimulation, Stem Cells, Proteomics |
Contact List
| Damarys Loew |
|---|
| contact affiliation | Head of the Curie Institute Mass Spectrometry Platform |
| contact email | damarys.loew@curie.fr |
| lab head | |
| Vanessa Masson |
|---|
| contact affiliation | Institut Curie - Centre de Recherche |
| contact email | vanessa.masson@curie.fr |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD059610 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD059610
- Label: PRIDE project
- Name: Harnessing hydrodynamics for high-yield production of extracellular vesicles from stem cells spheroids with specific cargo profiling
