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PXD059595

PXD059595 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDifferential protein expression analysis of western flower thrips +/- TSWV
DescriptionQuantitative LC/MS/MS was performed on 4 uL of each sample, using a nanoAcquity UPLC system (Waters Corp) coupled to a Thermo Orbitrap Fusion Lumos high resolution accurate mass tandem mass spectrometer (Thermo) equipped with a FAIMSPro device via a nanoelectrospray ionization source. Briefly, the sample was first trapped on a Symmetry C18 20 mm x 180 um trapping column (5 ul/min at 99.9/0.1 v/v water/acetonitrile), after which the analytical separation was performed using a 1.8 um Acquity HSS T3 C18 75 um x 250 mm column (Waters Corp.) with a 90-min linear gradient of 5 to 30% acetonitrile with 0.1% formic acid at a flow rate of 400 nanoliters/minute (nL/min) with a column temperature of 55C. Data collection on the Fusion Lumos mass spectrometer was performed for three difference compensation voltages (-40v, -60v, -80v). Within each CV, a data-dependent acquisition (DDA) mode of acquisition with a r=120,000 (m/z 200) full MS scan from m/z 375 - 1500 with a target AGC value of 4e5 ions was performed. MS/MS scans were acquired in the linear ion trap in rapid mode with a target AGC value of 1e4 and max fill time of 35 ms. The total cycle time for each CV was 0.66s, with total cycle times of 2 sec between like full MS scans. A 20s dynamic exclusion was employed to increase depth of coverage. The total analysis cycle time for each sample injection was approximately 2 hours
HostingRepositoryMassIVE
AnnounceDate2025-04-07
AnnouncementXMLSubmission_2025-04-07_07:36:06.454.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterErik Soderblom
SpeciesList scientific name: Thysanoptera; common name: thrips; NCBI TaxID: 30262;
ModificationListOxidation
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-01-09 07:38:46ID requested
12025-04-07 07:36:06announced
Publication List
no publication
Keyword List
submitter keyword: Thrips, proteomics, TSWV, DatasetType:Proteomics
Contact List
Dorith Rotenberg
contact affiliationNorth Carolina State University
contact emaildrotenb@ncsu.edu
lab head
Erik Soderblom
contact affiliationDuke University
contact emailes114@duke.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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