PXD059552

PXD059552 is an original dataset announced via ProteomeXchange.

Title	Cell Surface Biotinylation via Furan Crosslinking to Profile the Surfaceome
Description	The cellular surfaceome is crucial for cellular homeostasis and communication, with surface proteins acting as key receptors, transporters, and enzymes. However, comprehensive analysis of the surfaceome is challenging due to the hydrophobic nature and low abundance of membrane proteins, resulting underrepresented in mass spectrometry-based proteomic analyses. This study introduces a novel method for profiling the cell surfaceome using furan-biotin (FB) conjugates. We synthesized six FB compounds (FB1-FB6) and showed that FB1 reacts with lysine and cysteine in close proximity. FB1 exhibited the highest staining intensity and specificity for surface proteins upon oxidation. Protein pull-down assays confirmed the efficiency and reproducibility of FB1 in enriching surface proteins, outperforming traditional NHS-biotin methods by reducing nonspecific labeling of intracellular proteins. This method provides a robust tool for unbiased, high-specificity proteomic studies of cell surface dynamics, particularly under oxidative conditions, and opens new avenues for exploring cell surface interactions and protein modifications.
HostingRepository	PRIDE
AnnounceDate	2025-10-21
AnnouncementXML	Submission_2025-10-21_03:06:43.692.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Esperanza Fernandez
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-08 06:32:45	ID requested
⏵ 1	2025-10-21 03:06:44	announced

10.1007/978-1-0716-3457-8_2;

submitter keyword: proteomics, affinity purification,Furan-biotin, plasma membrane proteins, LC-MS/MS, surfaceome

Kris Gevaert
contact affiliation	VIB Center for Medical Biotechnology, VIB, B-9052 Ghent, Belgium Department of Biomolecular Medicine, Ghent University, B-9052 Ghent, Belgium
contact email	kris.gevaert@vib-ugent.be
lab head
Esperanza Fernandez
contact affiliation	VIB-UGent
contact email	esperanza.fernandez@vib-ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD059552

PRIDE project URI

• PRIDE
  1. PXD059552
     1. Label: PRIDE project
     2. Name: Cell Surface Biotinylation via Furan Crosslinking to Profile the Surfaceome