PXD059546 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | RNA antisense oligonucleotide pulldown coupled with mass spectrometry (MS) to identify C1QTNF1-AS1 protein interactors |
| Description | We used C1QTNF1-AS1 antisense oligonucleotide pulldown coupled with MS to identify protein interactors of lncRNA C1QTNF1-AS1 in human HCT116 cells. Five antisense oligonucleotides against C1QTNF1-AS1 and luciferase (negative control) were used for the pulldown. Three independent oligonucleotides (C1QTNF1-AS1 oligonucleotides 1, 3 and 5), efficiently retrieve C1QTNF1-AS1 and extracted proteins from these samples were compared using label-free (LFQ) Quantitative proteomics. C1QTNF1-AS1 oligonucleotides 2 and 4 were excluded from the analysis as they did not retrieve C1QTNF1-AS1. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-12 |
| AnnouncementXML | Submission_2026-03-12_14:49:37.649.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Faraz Mardakheh |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-01-08 04:31:42 | ID requested | |
| ⏵ 1 | 2026-03-12 14:49:38 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Antisense Oligo pulldown |
| RNA interactome |
| LC-MS/MS |
| Label-free Quantification |
Contact List
| Faraz Mardakheh |
|---|
| contact affiliation | Centre for Cancer Cell & Molecular Biology Barts Cancer Institute Queen Mary University of London John Vane Science Centre Charterhouse Square London EC1M 6BQ UK |
| contact email | f.mardakheh@qmul.ac.uk |
| lab head | |
| Faraz Mardakheh |
|---|
| contact affiliation | Department of Biochemistry,
University of Oxford |
| contact email | f.mardakheh@qmul.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD059546 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD059546
- Label: PRIDE project
- Name: RNA antisense oligonucleotide pulldown coupled with mass spectrometry (MS) to identify C1QTNF1-AS1 protein interactors
