PXD059541

PXD059541 is an original dataset announced via ProteomeXchange.

Title	Proteome analysis of biofilm extracellular vesicles produced by Granulicatella adiacens and Granulicatella elegans.
Description	Granulicatella adiacens and Granulicatella elegans are non-motile, non-spore-forming, facultatively anaerobic Gram-positive cocci. They are part of the normal oral flora but causes serious infections such as infective endocarditis. When oral bacteria accidentally enter the bloodstream due to transient tissue damage during dental procedures, they have the potential to attach to the endocardium or an equivalent surface of an indwelling prosthesis and cause infection. Many bacterial species produce extracellular vesicles (EVs) as a virulence strategy. The study of these EVs was initially limited to Gram-negative bacteria; however, their production was also observed in some Gram-positive bacteria. Recent research in our lab has found that G. adiacens produces abundant EVs in planktonic life style. However, EVs production by G. adiacens in biofilm mode is unknown. Therefore, the objective is to isolate and characterize EVs produced by this bacterium in biofilm mode. G. adiacens CCUG 27809 will be cultured on chocolate blood agar with 0.001% pyridoxal hydrochloride at 37 °C and 5% CO2 for 2 days. The EVs will be isolated using differential centrifugation and filtration protocol and then observed using electron scanning microscopy. Proteins in the biofilm EVs will be identified using mass spectrometry. Unraveling the EVs proteome composition of G. adiacens biofilms may help elucidate intricate virulence strategies of this species.
HostingRepository	PRIDE
AnnounceDate	2026-01-05
AnnouncementXML	Submission_2026-01-04_16:46:50.837.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maribasappa Karched
SpeciesList	scientific name: Granulicatella elegans; NCBI TaxID: NEWT:137732; scientific name: Granulicatella adiacens; NCBI TaxID: NEWT:46124; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-08 02:50:00	ID requested
⏵ 1	2026-01-04 16:46:51	announced

Karched M, Alkandari S, in Biofilm vs. Planktonic Lifestyle. Dent J (Basel), 13(12):(2025) [pubmed]

10.3390/dj13120557;

submitter keyword: Granulicatella, Biofilms, Infective endocarditis, Proteomics, Extracellular vesicles

Maribasappa Karched, PhD
contact affiliation	Department of Bioclinical Sciences College of Dentistry Kuwait University Kuwait.
contact email	maribasappa.karched@gmail.com
lab head
Maribasappa Karched
contact affiliation	Faculty of Dentistry Kuwait University
contact email	maribasappa.karched@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD059541

PRIDE project URI

• PRIDE
  1. PXD059541
     1. Label: PRIDE project
     2. Name: Proteome analysis of biofilm extracellular vesicles produced by Granulicatella adiacens and Granulicatella elegans.