PXD059522

PXD059522 is an original dataset announced via ProteomeXchange.

Title	Proteasomes Accumulate in the Plant Apoplast Where They Participate in Microbe-Associated Molecular Pattern (MAMP)-Triggered Pathogen Defense
Description	Akin to mammalian extracellular fluids, the plant apoplastic fluid (APF) contains a unique collection of proteins, RNAs, and vesicles that drive many physiological processes ranging from cell wall assembly to defense against environmental challenges. Using an improved method to enrich for the Arabidopsis APF, we better defined its composition and discovered that the APF harbors active proteasomes though microscopic detection, proteasome-specific activity and immunological assays, and mass spectrometry showing selective enrichment of the core protease. Functional analysis of extracellular (ex)-proteasomes revealed that they help promote basal pathogen defense through proteolytic release of microbe-associated molecular patterns (MAMPs) such as flg22 from bacterial flagellin that induce protective reactive-oxygen-species (ROS) bursts. Flagellin-triggered ROS is also strongly suppressed by the enigmatic Pseudomonas syringae virulence effector syringolin-A that blocks ex-proteasome activity. Collectively, we provide a deep catalog of apoplast proteins and evidence that ex-proteasomes participate in the evolving arms race between pathogens and their plant hosts.
HostingRepository	PRIDE
AnnounceDate	2025-01-08
AnnouncementXML	Submission_2025-01-07_20:23:45.875.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD059522
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kuo-En Chen
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	carbamoylated residue; monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-07 18:46:00	ID requested
⏵ 1	2025-01-07 20:23:46	announced

10.6019/PXD059522;

submitter keyword: Arabidopsis, syringolin-A, reactive oxygen species, pathogen defense, Pseudomonas syringae,Apoplast, proteasome, microbe-associated molecular patterns, flagellin

Richard D. Vierstra
contact affiliation	Department of Biology,Washington University in St. Louis
contact email	rdvierstra@wustl.edu
lab head
Kuo-En Chen
contact affiliation	Department of Biology, Washington University in St. Louis
contact email	kuo-en@wustl.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD059522
     1. Label: PRIDE project
     2. Name: Proteasomes Accumulate in the Plant Apoplast Where They Participate in Microbe-Associated Molecular Pattern (MAMP)-Triggered Pathogen Defense