PXD059516

PXD059516 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Comparative Proteomics of mHippoE-18 Cells Treated with Peptide Fraction from Naja mandalayensis Snake Venom
Description	Functional characterization of peptide fraction (PF) from snake venom has provided novel opportunities to investigate possible neuroprotective compounds relevant to pharmaceuticals. This study was performed to investigate the PF-mediated neuroprotection obtained from Naja mandalayensis snake venom, a member of the Elapidae family, using mHippoE-18 cell lines, in response to H2O2-induced oxidative stress. The comparative proteomic profiling identified 53 proteins with differential expression related to antioxidant action, catalysis, molecular function regulators, structural molecule activity, translation regulatory activity, ATP, and binding. The PF + H₂O₂ group indicated that protein expression is 6% upregulated, 4% downregulated, and 94% unchanged compared to the H₂O₂ group. Three significant proteins upregulated in the PF + H₂O₂ group, including elongation factor 2 (P58252), proteasome subunit alpha type (E9Q0X0), and E2 ubiquitin-conjugating enzyme (A0A338P786), suggested that PF-mediated neuroprotection happens through translational regulation and the degradation of defective proteins via the proteasome complex. Additionally, differential protein expression in PF changed the metabolism, protein synthesis, synaptic activity, and intracellular transport, suggesting that PF contains a rich mixture of bioactive peptides of pharmacological interest.
HostingRepository	jPOST
AnnounceDate	2025-01-08
AnnouncementXML	Submission_2025-01-07_09:31:09.687.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emidio Beraldo Neto
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-07 09:31:06	ID requested
⏵ 1	2025-01-07 09:31:10	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Naja mandalayensis, Bioactive peptide, Reactive oxygen, Neuroprotective

submitter keyword: Naja mandalayensis, Bioactive peptide, Reactive oxygen, Neuroprotective

Contact List

Emidio Beraldo
lab head
Emidio Beraldo Neto
contact affiliation	Butantan Institute
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003539/