PXD059514

PXD059514 is an original dataset announced via ProteomeXchange.

Title	Arg-C Ultra simplifies histone preparation for LC-MS/MS
Description	Using mammalian histone extract, we demonstrate that Arg-C Ultra facilitates histone preparation for LC-MS/MS. We show the performance of Arg-C Ultra in terms of digestion specificity, number of modified forms identified, and yield of quantitative information compared with standard Arg-C and trypsin digestion combined with chemical derivatization with trimethylacetic anhydride. We show that chemical derivatization at the peptide level, i.e., after Arg-C Ultra digestion, is still necessary to improve the quantification of short histone peptidoforms as well as positional isomers.
HostingRepository	PRIDE
AnnounceDate	2025-06-30
AnnouncementXML	Submission_2025-06-29_16:08:39.297.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gabriela Lochmanova
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	N6-methyl-L-lysine; N6; N6-acetyl-L-lysine; N6
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-07 08:02:12	ID requested
⏵ 1	2025-06-29 16:08:40	announced

10.1021/acs.analchem.5c02238;

Ryzhaya P, P, í, rek P, Zdr, á, hal Z, Lochmanov, á G, Arg-C Ultra Simplifies Histone Preparation for LC-MS/MS. Anal Chem, 97(24):12486-12492(2025) [pubmed]

submitter keyword: histone, Arg-C Ultra, trimethylacetic anhydride, LC-MS/MS, chemical derivatization

Zbynek Zdrahal
contact affiliation	Masaryk University
contact email	zdrahal@sci.muni.cz
lab head
Gabriela Lochmanova
contact affiliation	CEITEC-MU, Masaryk University, Brno
contact email	g.lochmanova@seznam.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD059514

PRIDE project URI

• PRIDE
  1. PXD059514
     1. Label: PRIDE project
     2. Name: Arg-C Ultra simplifies histone preparation for LC-MS/MS