PXD059507

PXD059507 is an original dataset announced via ProteomeXchange.

Title	Identification of RSRC2 interactome through Immunoprecipitation (IP) coupled with LC-MS/MS-based protein analysis
Description	We immunoprecipitate (IP) RSRC2 from HCT116 cells in the presence or absence of RNase A/T1 mix to identify RNA-mediated and RNA-independent RSRC2 protein-protein interaction networks. Four biological replicates were performed for each IP-MS in cells +/- RNase A/T1 Mix (EN0551, ThermoFisher) using IgG and RSRC2 antibodies. Extracted proteins from RNase A/T1 treated and untreated cells (4 replicates per condition) were compared using label-free (LFQ) Quantitative proteomics.
HostingRepository	PRIDE
AnnounceDate	2026-03-12
AnnouncementXML	Submission_2026-03-12_15:26:12.119.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Faraz Mardakheh
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-07 06:22:53	ID requested
⏵ 1	2026-03-12 15:26:12	announced

Dataset with its publication pending

submitter keyword: Label-free Quantification

Interactome analysis

RSRC2

IP-MS

Faraz Mardakheh
contact affiliation	Centre for Cancer Cell & Molecular Biology, Barts Cancer Institute, Queen Mary University of London, John Vane Science Centre, Charterhouse Square, London EC1M 6BQ, UK
contact email	f.mardakheh@qmul.ac.uk
lab head
Faraz Mardakheh
contact affiliation	Department of Biochemistry, University of Oxford
contact email	f.mardakheh@qmul.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD059507

PRIDE project URI

• PRIDE
  1. PXD059507
     1. Label: PRIDE project
     2. Name: Identification of RSRC2 interactome through Immunoprecipitation (IP) coupled with LC-MS/MS-based protein analysis