PXD059486

PXD059486 is an original dataset announced via ProteomeXchange.

Title	Preliminary investigation into the impact of GGDEF family gene deletion on the phenotype of Aeromonas hydrophila
Description	This study took Aeromonas hydrophila as an example to study the GGDEF family gene and explore its biological function in Aeromonas hydrophila and its influence on c-di-GMP concentration. In order to systematically study the biological function of GGDEF family genes, 36 strains (54 strains screened by whole genome sequencing) with gene deletion mutation were first constructed, and then phenotypes such as biofilm, crawling and extracellular protease activity were detected. The concentration of c-di-GMP in aeromonas hydrophila was determined by HPLC. At the same time, the deletion strains with GGDEF domain that had a significant effect on the physiological function of Aeromonas hydrophila were screened for mass spectrometry identification, so as to explore the possible molecular mechanism of GGDEF domain protein affecting the physiological function.
HostingRepository	iProX
AnnounceDate	2025-01-06
AnnouncementXML	Submission_2025-01-06_18:52:12.895.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiangmin Lin
SpeciesList	scientific name: Aeromonas hydrophila subsp. hydrophila ATCC 7966; NCBI TaxID: 380703;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-06 18:51:55	ID requested
⏵ 1	2025-01-06 18:52:13	announced

Dataset with its publication pending

submitter keyword: Aeromonas hydrophila,phenotype,biofilm,antibiotic

Xiangmin Lin
contact affiliation	Fujian Agriculture and Forestry University
contact email	313461278@qq.com
lab head
Xiangmin Lin
contact affiliation	Fujian Agriculture and Forestry University
contact email	313461278@qq.com
dataset submitter

iProX dataset URI