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PXD059447

PXD059447 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleNovel kethoxal derivatives-assisted identification of single-stranded RNA-binding proteins
DescriptionThis project introduces the kethoxal-assisted single-stranded RNA interactome capture (KASRIC) strategy to enable transcriptome-wide identification of single-stranded RBPs (ssRBPs). The kethoxal probe selectively modifies unpaired guanine residues in RNA single-stranded regions, thereby blocking ssRBP interaction near these sites. After RNA-protein crosslinking and oligo(dT) bead-mediated capture, enriched proteins are analyzed using DIA proteomics. Proteins with significantly down-regulated abundance in kethoxal-treated groups (N-Block and A-Block) compared to the crosslinked control group (cCL) are identified as candidate ssRBPs.
HostingRepositoryiProX
AnnounceDate2025-01-03
AnnouncementXMLSubmission_2025-01-05_19:26:06.035.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterXi Chen
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-01-05 19:25:53ID requested
⏵ 12025-01-05 19:26:06announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: single-stranded RNA-binding proteins
RNA-binding proteins
ssRBPs
RBPs
DIA
Contact List
Xiaocheng Weng
contact affiliationWuhan University
contact emailxcweng@whu.edu.cn
lab head
Xi Chen
contact affiliationWuhan University
contact email2018302030024@whu.edu.cn
dataset submitter
Full Dataset Link List
iProX dataset URI

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