PXD059427

PXD059427 is an original dataset announced via ProteomeXchange.

Title	MS-based sequence of anti-RSV-F monoclonal antibody 131-2a
Description	The Respiratory Syncytial Virus (RSV) Fusion (F) protein is a major target of antiviral antibodies following natural infection and vaccination and responsible for mediating fusion between the viral envelope and the host membrane. The fusion process is driven by a large-scale conformational change in F, switching irreversibly from the metastable prefusion state to the stable postfusion conformation. Previous research has identified six distinct antigenic sites in RSV-F, termed sites Ø, I, II, III, IV, and V. Of these, only antigenic site I is fully specific to the postfusion conformation of F. A monoclonal antibody 131-2a that specifically targets postfusion F has been widely used as a research tool to probe for postfusion F and to define antigenic site I in serological studies, yet its sequence and precise epitope have remained unknown. Here we use mass spectrometry-based de novo sequencing of 131-2a to reverse engineer a recombinant product and study the epitope to define antigenic site I with molecular detail, revealing the structural basis for the antibody’s specificity towards postfusion RSV-F.
HostingRepository	PRIDE
AnnounceDate	2025-07-22
AnnouncementXML	Submission_2025-07-22_04:57:49.079.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joost Snijder
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-03 12:48:27	ID requested
⏵ 1	2025-07-22 04:57:49	announced

Dataset with its publication pending

submitter keyword: antibody, respiratory syncytial virus, de novo

Joost Snijder
contact affiliation	Utrecht University
contact email	j.snijder@uu.nl
lab head
Joost Snijder
contact affiliation	Utrecht University
contact email	joost.snijder@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD059427

PRIDE project URI

• PRIDE
  1. PXD059427
     1. Label: PRIDE project
     2. Name: MS-based sequence of anti-RSV-F monoclonal antibody 131-2a