PXD059420

PXD059420 is an original dataset announced via ProteomeXchange.

Title	Immunoprecipitation of STING/ADSL and mass spectrometry analysis
Description	BT-549 cells with or without expression of Flag-STING were treated with or without hypoxia for 6 h. An immunoprecipitation assay was performed using anti-Flag antibody, and immunoprecipitates of Flag-STING were eluted with Flag peptide, separated using SDS-PAGE and stained with Coomassie Brilliant Blue. Selected peptide hits of proteins associated with Flag-STING identified through mass spectrometry are shown.Bacterially purified His-ADSL proteins on Ni-NTA agarose beads were incubated with or without active GST-IKKβ in the presence of ATP for an in vitro kinase assay;Then the protein samples were digested using GluC and analyzed using LC-MS/MS with the Orbitrap Elite mass spectrometer.
HostingRepository	PRIDE
AnnounceDate	2025-02-15
AnnouncementXML	Submission_2025-02-15_07:32:03.598.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	yuran duan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-03 07:23:59	ID requested
⏵ 1	2025-02-15 07:32:03	announced

10.1038/S41556-025-01627-8;

submitter keyword: mass spectrometry,protein-protein interaction,phopshorylation

Daqian Xu
contact affiliation	Zhejiang University
contact email	xudaqian@zju.edu.cn
lab head
yuran duan
contact affiliation	Institute of Translational Medicine, Zhejiang University School of Medicine, Zhejiang University
contact email	yuranduan@zju.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD059420

PRIDE project URI

• PRIDE
  1. PXD059420
     1. Label: PRIDE project
     2. Name: Immunoprecipitation of STING/ADSL and mass spectrometry analysis