PXD059360
PXD059360 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | TRPM7 is cleaved by calpain1 to release the kinase to regulate synaptic density and plasticity in a cell compartment-specific manner |
| Description | The magnesium, zinc and calcium ion channel TRPM7 is a channel-enzyme that has been implicated in synaptic and cognitive functions in the mammalian brain. How TRPM7 translates neuronal activity into activation of signaling pathways to regulate such functions remains largely unknown. Here, we found that calpain1 cleaves TRPM7 in neuronal activity-/calcium-dependent way, to release a C-terminal fragment containing the magnesium-dependent kinase domain (M7CK). Spatial proteomics, biochemical, cellular, transcriptomic and protein expression studies revealed that M7CK is translocated to nucleus to activate MEF2C to regulate expression of genes such as Prickle2 (a zinc binding protein) to regulate synapse density. Meanwhile, M7CK is translocated to dendrites to activate targets such as the mTOR-signaling member RPS6 to regulate synthesis of proteins including zinc transporter1 to control synaptic plasticity. Results provide insights of how TRPM7 activates distinct signaling pathways regulating distinct cellular functions necessary for supporting synaptic and cognitive functions in cell compartment-specific manner. |
| HostingRepository | iProX |
| AnnounceDate | 2024-12-31 |
| AnnouncementXML | Submission_2025-05-20_21:45:14.921.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Bingqing Guo |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-01-01 20:45:14 | ID requested | |
| ⏵ 1 | 2025-05-20 21:45:15 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: TRPM7, kinase domain M7CK, cleavage, cell compartment-specific manner, spatial proteomic |
Contact List
| Nashat Abumaria | |
|---|---|
| contact affiliation | Fudan University |
| contact email | abumaria@fudan.edu.cn |
| lab head | |
| Bingqing Guo | |
| contact affiliation | Fudan University |
| contact email | gbqing888@126.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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