PXD059286

PXD059286 is an original dataset announced via ProteomeXchange.

Title	Multi-compartment quantitative proteomics revealing potential mechanisms underlying the treatment effects of mesenchymal-derived extracellular vesicles in a monkey model of cortical injury
Description	Cortical injury frequently causes motor dysfunction, significantly impairing activities of daily living, and currently, there are no effective therapeutic options available. Previous studies demonstrated that intravenous infusion of mesenchymal stromal cell-derived extracellular vesicles (MSC-EVs) exerts beneficial effects following cortical injury in primary motor cortex via reducing injury-induced microglial neuroinflammation, neuronal excitotoxicity, synapse loss, oligodendrocyte damage and myelination deficits in the rhesus monkey. However, the specific molecules within MSC-EVs responsible for these therapeutic effects and the underlying molecular mechanisms remain largely unexplored. In this study, employing quantitative proteomics strategy, we first identified the protein composition of MSC-EVs, then analyzed the global protein expression changes associated with cortical injury and treatment effect in brain tissue, cerebrospinal fluid (CSF), and plasma samples from monkeys following cortical injury. A total of 4124/1241/431 monkey proteins were reliably quantified in tissue/CSF/plasma samples, respectively. Further informatics analysis revealed not only the enriched biological processes aligned with previous findings, but also several potential treatment markers. This first-time comprehensive investigation of quantitative proteomics in multi-compartment samples from rhesus monkey enhanced our understanding of the treatment effects of MSC-EVs, paving the way for novel treatment and biomarker discovery.
HostingRepository	PRIDE
AnnounceDate	2026-06-15
AnnouncementXML	Submission_2026-06-14_21:31:05.868.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shuo Qian
SpeciesList	scientific name: Macaca mulatta (Rhesus macaque); NCBI TaxID: NEWT:9544;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-28 17:02:47	ID requested
⏵ 1	2026-06-14 21:31:06	announced

10.1021/acs.jproteome.5c00521;

Qian S, McCann RP, Shen S, Zhu X, Tsillas CM, Xin H, Wang M, Zhang Y, Zhang ZG, Chopp M, Medalla M, Moore TL, Qu J, Multicompartment Quantitative Proteomics Revealing Potential Mechanisms Underlying the Treatment Effects of Mesenchymal-Derived Extracellular Vesicles in a Monkey Model of Cortical Injury. J Proteome Res, 24(11):5664-5682(2025) [pubmed]

submitter keyword: mesenchymal stromal cell-derived extracellular vesicles, Cortical Injury,Rhesus Macaque, Brain Tissue, Plasma, CSF, LC-MS/MS

Jun Qu
contact affiliation	Department of Pharmaceutical Sciences, University at Buffalo, Buffalo, NY, USA
contact email	junqu@buffalo.edu
lab head
Shuo Qian
contact affiliation	Department of Cell Stress Biology, Roswell Park Comprehensive Cancer Center
contact email	sqian@buffalo.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD059286

PRIDE project URI

• PRIDE
  1. PXD059286
     1. Label: PRIDE project
     2. Name: Multi-compartment quantitative proteomics revealing potential mechanisms underlying the treatment effects of mesenchymal-derived extracellular vesicles in a monkey model of cortical injury