PXD059253

PXD059253 is an original dataset announced via ProteomeXchange.

Title	S-nitrosylation mediated by Staphylococcus aureus-derived nitric oxide modulates virulence and host local inflammatory response
Description	Staphylococcus aureus has become a critical source of community and nosocomial infections. Due to the widespread use of antibiotics, S. aureus has developed resistance to multiple antibiotics, making clinical treatment more difficult. Therefore, it is of significant clinical relevance to investigate the pathogenic mechanism of S. aureus and identify new therapeutic targets. Bacterial endogenous signaling molecule nitric oxide (NO) plays an important role in regulating pathogenicity, but the mechanism remains to be further explored. Our results have shown that S-nitrosylation mediated by S. aureus-derived NO has significant effects on the virulence of bacterial pathogen and the activity of macrophages, and GntR and Hsp90 are the key modification targets in S. aureus and macrophages, respectively. In order to explore the pathogenesis of S. aureus mediated by NO，we hypothesized that endogenous NO regulates exopolysaccharide (EPS) synthesis of S. aureus through mediating S-nitrosylation of GntR, thus enhancing the virulence and regulates endoplasmic reticulum stress (ERS) by mediating Hsp90 S-nitrosylation, thereby modulating M1/M2 polarization of macrophage and host local inflammatory response, and ultimately achieving the pathogenic process of S. aureus. The hypothesis is validated through histological and molecular biology approaches, combining in vitro and in vivo experimental schemes. The research is expected to provide a new perspective for understanding the pathogenic mechanism of S. aureus and potentially new targets for future antimicrobial therapies.
HostingRepository	iProX
AnnounceDate	2024-12-25
AnnouncementXML	Submission_2024-12-25_18:31:42.406.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yujie Li
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090; scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-25 18:31:24	ID requested
⏵ 1	2024-12-25 18:31:42	announced

Dataset with its publication pending

submitter keyword: Pathogenic mechanism, Host, Staphylococcus aureus, Nitric oxide, S-nitrosylation

Baolin Sun
contact affiliation	University of Science and Technology of China
contact email	sunb@ustc.edu.cn
lab head
Yujie Li
contact affiliation	University of Science and Technology of China
contact email	lyj2020@ustc.edu.cn
dataset submitter

iProX dataset URI