PXD059243

PXD059243 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	SH2B1 Promotes Apoptosis in Diabetic Cataract via p38 MAPK Pathway
Description	Diabetic patients face an increased risk of developing cataracts, with unclear mechanisms. Our study illuminates these mechanisms by identifying differentially expressed proteins in the lens anterior capsule of diabetic cataract (DC) and age-related cataract patients using quantitative proteomics. We found SH2B1 to be crucial in DC progression. Reduced SH2B1 expression was confirmed through PCR and Western blotting in patient samples, diet-induced obese mice, and high-glucose (HG)-cultured human lens epithelial cells. Under HG conditions, cell proliferation decreased, while migration and apoptosis, alongside changes in Bcl2 and caspase 3 expression, increased. Overexpressing SH2B1 alleviated these changes and influenced the p38 MAPK signaling pathway. This suggests SH2B1 and the p38 MAPK pathway as significant in DC pathogenesis and potential therapeutic targets. Clinically, this could lead to therapies aimed at halting or slowing DC progression.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_06:02:44.632.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yun-E Zhao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-25 01:44:29	ID requested
⏵ 1	2025-05-07 06:02:45	announced

Publication List

10.1016/j.isci.2024.111735;
Jiang X, Xu L, Xu B, Peng H, Yang T, Zhao Y, Wu N, Zhao YE, SH2B1 promotes apoptosis in diabetic cataract via p38 MAPK pathway. iScience, 28(2):111735(2025) [pubmed]

10.1016/j.isci.2024.111735;

Jiang X, Xu L, Xu B, Peng H, Yang T, Zhao Y, Wu N, Zhao YE, SH2B1 promotes apoptosis in diabetic cataract via p38 MAPK pathway. iScience, 28(2):111735(2025) [pubmed]

Keyword List

submitter keyword: Ophthalmology
protein interaction network,;high-fat diet
diabetic cataract
cell culture, proteomics, oxidative stress,;high-glucose environment
autophagy
epithelial–mesenchymal transition

submitter keyword: Ophthalmology

protein interaction network,;high-fat diet

diabetic cataract

cell culture, proteomics, oxidative stress,;high-glucose environment

autophagy

epithelial–mesenchymal transition

Contact List

Yun-e Zhao
contact affiliation	Eye Hospital of Wenzhou Medical University at Hangzhou, 618 East Fengqi Road, Hangzhou
contact email	zyehzeye@126.com
lab head
Yun-E Zhao
contact affiliation	Eye Hospital of Wenzhou Medical University at Hangzhou
contact email	zyehzeye@126.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD059243
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD059243

PRIDE project URI

Repository Record List

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