PXD059234

PXD059234 is an original dataset announced via ProteomeXchange.

Title	Analysis of O-glycans by oxidative release combined with 3-nitrophenylhydrazine derivatization
Description	Glycosylation profiling is an effective methodology for achieving a comprehensive understanding of glycoproteins and their alterations in a multitude of pathological conditions. However, in comparison to N-glycosylation, O-glycosylation presents significant challenges in terms of both qualitative and quantitative mass spectrometric analysis. A recently developed oxidative release protocol enables the selective formation of O-glycans containing a carboxyl group derived from the amino acid residue. In this study, 3-nitrophenylhydrazine was used to derivatized the common carboxyl group in a mild hydrophilic solution.
HostingRepository	iProX
AnnounceDate	2024-12-24
AnnouncementXML	Submission_2025-06-29_22:58:34.749.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhenghu Min
SpeciesList	scientific name: Bos taurus; NCBI TaxID: 9913; scientific name: Equus caballus; NCBI TaxID: 9796; scientific name: Carassius auratus ssp. 1 MT-2012; NCBI TaxID: 1220728;
ModificationList	O-glycosyl-L-serine; O-glycosyl-L-threonine
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-24 19:56:19	ID requested
⏵ 1	2025-06-29 22:58:35	announced

Dataset with its publication pending

submitter keyword: O-glycosylation, O-glycan, NaClO, oxidation release, 3-NPH, derivatization, LC-MS

Qiwei Zhang
contact affiliation	School of Optoelectronic Materials & Technology, Jianghan University
contact email	keewezhang@jhun.edu.cn
lab head
Zhenghu Min
contact affiliation	School of Environment and Health, Jianghan University
contact email	mzh@stu.jhun.edu.cn
dataset submitter

iProX dataset URI