PXD059194

PXD059194 is an original dataset announced via ProteomeXchange.

Title	Quantitative reactivity profiling of functional arginines in the human proteome
Description	Arginine, a nucleophilic amino acid crucial for protein structure and function, plays a key role in various biological processes like enzyme catalysis and macromolecular interactions. Despite challenges in selectively targeting its less reactive guanidine group, we developed a novel probe featuring phenyl glyoxal, AP-1, which exhibited exceptional chemical selectivity and reactivity towards arginines. Utilizing activity-based protein profiling (ABPP), we explored the human proteome across four cancer cell lines, quantifying about 17,000 arginines. This analysis led us to identify several previously unreported key hyperreactive arginines, including R43 of PKM, R171 of LDHA, R172 of LDHB, R341 of CKB, R168 of EIF4A1, and R118 of FUBP1, that play crucial roles in protein functions, highlighting the importance of these residues in mediating enzyme activity or macromolecular interactions and their therapeutic potential. Notably, mutation of CKB’s R341 can inhibit cell proliferation and migration via downregulating energy supply. Additionally, we introduced ArGO-LDHA-1, a covalent inhibitor targeting hyperreactive arginines of LDHA, demonstrating covalent binding properties and potential to enhance chemotherapy efficacy. This work not only validated the significance of numerous arginines with profound biological functions but also introduced a novel, efficient platform for large-scale arginine reactivity profiling.
HostingRepository	PRIDE
AnnounceDate	2024-12-24
AnnouncementXML	Submission_2024-12-24_11:35:58.932.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wenbo Zhao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-23 15:55:20	ID requested
⏵ 1	2024-12-24 11:35:59	announced

Dataset with its publication pending

submitter keyword: arginine, quantitative profiling,ABPP

Xiaoguang Lei
contact affiliation	College of chemistry and molecular engineering, Peking University
contact email	xglei@pku.edu.cn
lab head
Wenbo Zhao
contact affiliation	Peking University
contact email	zhaowb@stu.pku.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/12/PXD059194

PRIDE project URI

• PRIDE
  1. PXD059194
     1. Label: PRIDE project
     2. Name: Quantitative reactivity profiling of functional arginines in the human proteome