PXD059189

PXD059189 is an original dataset announced via ProteomeXchange.

Title	Uukuniemi virus infection causes a pervasive remodelling of the RNA-binding proteome in tick cells
Description	Cellular RNA-binding proteins (RBPs) are pivotal for the viral lifecycle, mediating key host-virus interactions that promote or repress virus infection. While these interactions have been largely studied in the vertebrate host, no comprehensive analyses of protein-RNA interactions occurring in cells of arbovirus vectors, in particular ticks, have been performed to date. Here we systematically identify the responses of the RNA-binding proteome (RBPome) to infection with a prototype bunyavirus (Uukuniemi virus; UUKV) in tick cells and discovered changes in RNA-binding activity for 283 proteins. In an orthogonal approach, we analysed the composition of the viral ribonucleoprotein by immunoprecipitation of UUKV nucleocapsid protein (N) in infected cells. We found many tick RBPs that are regulated by UUKV infection and associate with viral nucleocapsid protein complexes. We confirmed experimentally that these RBPs impact UUKV infection. This includes the tick homolog of topoisomerase 3B (TOP3B), a protein able to manipulate the topology of RNA, which showed an effect on viral particle production. Our data thus reveals the first protein-RNA interaction map for infected tick cells.
HostingRepository	PRIDE
AnnounceDate	2025-07-29
AnnouncementXML	Submission_2025-07-29_01:17:55.392.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wael Kamel
SpeciesList	scientific name: Ixodes scapularis (Black-legged tick) (Deer tick); NCBI TaxID: 6945;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-23 08:20:10	ID requested
⏵ 1	2025-07-29 01:17:55	announced

10.1371/JOURNAL.PPAT.1013393;

submitter keyword: RNA-binding proteome, Uukuniemi virus

Benjamin Brennan
contact affiliation	Senior Research Fellow at MRC University of Glasgow Centre for Virus Research
contact email	Ben.Brennan@glasgow.ac.uk
lab head
Wael Kamel
contact affiliation	Glasgow university
contact email	wael.s.kamel@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD059189
     1. Label: PRIDE project
     2. Name: Uukuniemi virus infection causes a pervasive remodelling of the RNA-binding proteome in tick cells