PXD059178

PXD059178 is an original dataset announced via ProteomeXchange.

Title	CRISPR screening reveals that RNA helicase DDX41 triggers ribosome biogenesis and cancer progression through R-loop-mediated RPL/RPS transcription
Description	The RNA helicase DDX41 is a DEAD-box helicase that is well known as a virus sensor in dendritic cells and a tumor suppressor that is frequently mutated in myeloid neoplasms. However, the functions and relevance of DDX41 in solid tumors remain largely unexplored. In this study, through in vivo CRISPR screening, we demonstrated that DDX41 is highly expressed in various solid tumor types and promotes tumorigenicity in liver cancer. Mechanistically, DDX41 facilitates R-loop processing and accelerates the transcription of RPL/RPS genes, thereby promoting ribosome biogenesis and protein synthesis. Additionally, elevated DDX41 levels increase liver cancer cell sensitivity to protein synthesis inhibitors; treatment with homoharringtonine (HHT) significantly inhibits tumor growth in DDX41-overexpressing liver cancer models. Taken together, the results of this study highlight that DDX41 acts as an oncogene in liver cancer and suggest that protein synthesis inhibition may be a promising therapy for liver cancers with high DDX41 expression.
HostingRepository	iProX
AnnounceDate	2024-12-23
AnnouncementXML	Submission_2025-08-20_19:20:02.848.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hongquan Li
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-23 00:16:43	ID requested
⏵ 1	2025-08-20 19:20:03	announced

Li H, He Y, Jiang J, Liu Z, Liu Y, Shi Q, Ding J, Li H, Sun W, Hu X, Chen Z, He X, CRISPR screening reveals that RNA helicase DDX41 triggers ribosome biogenesis and cancer progression through R-loop-mediated RPL/RPS transcription. Nat Commun, 16(1):7409(2025) [pubmed]

submitter keyword: DDX41, R-loop structure, RPL/RPS, ribosome biogenesis, protein synthesis rate

Xianghuo He
contact affiliation	Fudan University Shanghai Cancer Center
contact email	xhhe@fudan.edu.cn
lab head
Hongquan Li
contact affiliation	Fudan University Shanghai Cancer Center
contact email	hongquan@fudan.edu.cn
dataset submitter

iProX dataset URI