PXD059170

PXD059170 is an original dataset announced via ProteomeXchange.

Title	High-resolution mass spectrometry for identifying phosphorylation sites of the metabotropic glutamate receptor mGlu3
Description	Metabotropic glutamate receptors (mGlus) modulate synaptic transmission in the central nervous system, while β-arrestins (βarrs) mediate the desensitization and internalization of activated mGlus. We report two structures of the metabotropic glutamate receptor subtype 3 (mGlu3) coupled to βarr1 with stoichiometries of 2:1 and 2:2, respectively. Guided by the density map, we identified three phosphorylation sites (pSer857, pSer859 and pThr860), which follow the P-X-P-P type phosphorylation pattern. To verify the phosphorylation sites observed in the cryo-EM structure, we conducted mass spectrometry (MS) experiment to identify phosphorylation sites on mGlu3 mediated by GRK2. The results show that three phosphorylation sites (pSer857, pSer859 and pThr860) were identified in mass spectra, which is consistent with the findings from our cryo-EM density map.
HostingRepository	PRIDE
AnnounceDate	2025-02-25
AnnouncementXML	Submission_2025-02-25_00:01:12.740.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tianlei Wen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-22 22:34:47	ID requested
⏵ 1	2025-02-25 00:01:13	announced

10.1038/S41589-025-01858-8;

submitter keyword: LC-MSMS, membrane protein,Human mGlu3, purified protein complex

Yuequan Shen
contact affiliation	State Key Laboratory of Medicinal Chemical Biology and Frontiers Science Center for Cell Responses, College of Life Sciences, Nankai University, Tianjin, China
contact email	yshen@nankai.edu.cn
lab head
Tianlei Wen
contact affiliation	Nankai University
contact email	yzlh053722@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD059170

PRIDE project URI

• PRIDE
  1. PXD059170
     1. Label: PRIDE project
     2. Name: High-resolution mass spectrometry for identifying phosphorylation sites of the metabotropic glutamate receptor mGlu3