PXD059168

PXD059168 is an original dataset announced via ProteomeXchange.

Title	The lactylome of mice granulosa cells
Description	Follicular GCs were obtained through intraperitoneal injections of 10, 5, 2, and 2 IU FSH at 12 h intervals. For the enrichment of Kla-modified peptides, the tryptic peptides were solubilized in NETN buffer (1 mM EDTA, 50 mM Tris–HCl, 100 mM NaCl, 0.5% NP-40, pH 8.0) and then incubated with prewashed beads (PTM Bio) at 4 °C for 12 h. Elution of the bound peptides was achieved using trifluoroacetic acid (0.1%). The eluted peptides were subsequently vacuum-dried and subjected to desalting using C18 ZipTips (Millipore) prior to LC‒MS/MS analysis.
HostingRepository	PRIDE
AnnounceDate	2025-09-29
AnnouncementXML	Submission_2025-09-28_16:17:52.149.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gang Wu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	lactic acid
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-22 22:32:15	ID requested
⏵ 1	2025-09-28 16:17:53	announced

10.1093/nar/gkaf882;

Wu G, Chen M, He T, Pan Y, Li C, Liu Z, Li H, Sheng Y, Dai W, Shen M, Liu H, Lactylation of CREB is required for FSH-induced proliferation and differentiation of ovarian granulosa cells. Nucleic Acids Res, 53(17):(2025) [pubmed]

submitter keyword: The lactylome of mice granulosa cells

GANG WU
contact affiliation	Nanjing Agricultural University,
contact email	2022205011@stu.njau.edu.cn
lab head
Gang Wu
contact affiliation	Nanjing Agricultural University
contact email	2022205011@stu.njau.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD059168

PRIDE project URI

• PRIDE
  1. PXD059168
     1. Label: PRIDE project
     2. Name: The lactylome of mice granulosa cells