PXD059165

PXD059165 is an original dataset announced via ProteomeXchange.

Title	Research on Downstream Binding Proteins of PlexinB1 in Human Brain Vascular Pericytes
Description	Age-related macular degeneration (AMD) is a prevalent neuroinflammation condition and the leading cause of irreversible blindness among the elderly population. Smoking significantly increases AMD risk, yet the mechanisms remain unclear. Here, we investigated the role of Sema4D-PlexinB1 axis in the progression of AMD, in which Sema4D-PlexinB1 is highly activated by smoking. Using patient-derived samples and mouse models, we discovered that smoking increased the presence of Sema4D on the surface of CD8+ T cells that migrated into the choroidal neovascularization (CNV) lesion via CXCL12-CXCR4 axis and interacted with its receptor PlexinB1 on choroidal pericytes. This led to ROR2-mediated PlexinB1 phosphorylation and pericytes activation, hence disrupted vascular homeostasis and promoted neovascularization. Inhibition of Sema4D reduced CNV and improved the benefit of anti-VEGF treatment. In conclusion, this study unveils the molecular mechanisms through which smoking exacerbates AMD pathology, and presents a potential therapeutic strategy by targeting Sema4D to augment current AMD treatments.
HostingRepository	PRIDE
AnnounceDate	2025-01-26
AnnouncementXML	Submission_2025-01-26_06:19:33.437.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	凯 何
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-22 22:24:05	ID requested
⏵ 1	2025-01-26 06:19:33	announced

Dataset with its publication pending

submitter keyword: Sema4D PlexinB1 ROR2 Pericyte

Kai He
contact affiliation	Tianjin Medical University General Hospital
contact email	hekai1994@yeah.net
lab head
凯 何
contact affiliation	Department of Ophthalmology, Tianjin Medical University General Hospital
contact email	hekai1994@yeah.net
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD059165
     1. Label: PRIDE project
     2. Name: Research on Downstream Binding Proteins of PlexinB1 in Human Brain Vascular Pericytes