PXD059154

PXD059154 is an original dataset announced via ProteomeXchange.

Title	Shotgun proteomics analysis by LC-ESI-MS/MS in pregnant women with excessive gingival bleeding
Description	Pregnancy induces significant physiological, immunological, and hormonal changes, particularly elevated levels of estrogen and progesterone, which increase susceptibility to periodontal inflammation. Gingivitis, the early stage of periodontal inflammation, is primarily triggered by dental biofilm accumulation. This study aimed to investigate the pathophysiological mechanisms of gingivitis during pregnancy by analyzing the salivary proteomic profile of pregnant women with and without excessive gingival bleeding. Pregnant women were categorized into two groups: those with excessive gingival bleeding on probing (BOP > 50%; G1, n = 9) and those without generalized gingivitis (BOP < 30%; G2, n = 9). A comprehensive full-mouth periodontal examination was performed. Unstimulated whole-mouth saliva samples were collected and individually processed using Nano Liquid Chromatography Electron Spray Ionization Tandem Mass Spectrometry (nLC- ESI-MS/MS). Proteins were considered significantly downregulated at p < 0.05 and upregulated at 1-p > 0.95. Proteomic analysis identified 187 salivary proteins, with 75 shared between groups. Among these, 60 proteins were upregulated and 7 were downregulated in G1, with key upregulated proteins including Protein S100-A9 (16-fold increase), Neutrophil Defensins 1 and 3 (7-fold increase), Protein S100-A8 (5-fold increase), Beta-2-Microglobulin (4-fold increase), and several immunoglobulin isoforms, while Histatin-3 was notably the only protein downregulated by more than 2-fold. Gene Ontology analysis revealed significant enrichment in processes related to antimicrobial humoral response, bacterial defense mechanisms, and immune regulation, reflecting the inflammatory state. These findings provide novel insights into the salivary proteomic alterations associated with gingivitis in pregnancy, emphasizing the heightened antimicrobial and immune activity linked to excessive gingival inflammation.
HostingRepository	PRIDE
AnnounceDate	2026-02-17
AnnouncementXML	Submission_2026-02-17_09:54:44.114.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gerson Foratori Junior
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Xevo G2 Q-Tof; nanoACQUITY UPLC

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-22 20:41:28	ID requested
⏵ 1	2026-02-17 09:54:44	announced

10.1111/ODI.70127;

submitter keyword: None

Gerson Aparecido
contact affiliation	Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo
contact email	gerson.foratori@usp.br
lab head
Gerson Foratori Junior
contact affiliation	Pediatric Dentistry, Orthodontics and Public Health, Bauru School of Dentistry, University of São Paulo
contact email	gerson.foratori@usp.br
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD059154

PRIDE project URI

• PRIDE
  1. PXD059154
     1. Label: PRIDE project
     2. Name: Shotgun proteomics analysis by LC-ESI-MS/MS in pregnant women with excessive gingival bleeding