PXD059153

PXD059153 is an original dataset announced via ProteomeXchange.

Title	Proteomic and Behavioral Study of the Anxiolytic Effects of GABA in a Chronic Restraint Stress-Induced Anxiety Model
Description	This study aimed to investigate the anxiolytic effects of GABA administration and its underlying molecular mechanisms in a chronic restraint stress (CRS)-induced anxiety model in C57BL/6J mice. CRS mice were established by placing the animals in well-ventilated 50 ml centrifuge tubes for 2 hours daily over 14 consecutive days, while control mice were housed under standard conditions. Behavioral assessments, including the elevated plus maze (EPM) test and open field test (OFT), were performed on days 15 and 16, respectively. GABA was administered intragastrically at various doses throughout the experiment, and prefrontal cortex (PFC) and hippocampal tissues were harvested on day 17 for further proteomic and biochemical analyses. Behavioral results demonstrated that GABA treatment exerted dose-dependent anxiolytic effects, as evidenced by increased time and entries in the open arms in the EPM and increased time and distance spent in the center area in the OFT. Furthermore, GABA treatment significantly elevated reduced GABA levels in the PFC. To elucidate the molecular mechanisms of GABA’s anxiolytic effects, a 4D-label-free quantitative proteomics approach was employed. Principal component analysis (PCA) and differential protein analyses revealed that CRS induced substantial proteomic alterations in the hippocampus, while GABA treatment partially reversed these changes. Functional clustering (Mfuzz) and gene ontology (GO) enrichment analysis identified critical pathways involved in the “humoral immune response,” “complement activation,” and “acute inflammatory response.” Key proteins such as C3, Cfh, C4b, and Cfi were found to overlap across these pathways, highlighting their involvement in CRS-induced neuroimmune dysfunction and GABA-mediated rescue effects. This integrated analysis provides valuable insights into the behavioral and neuroimmune mechanisms through which GABA alleviates anxiety-like behaviors, shedding light on its potential as a therapeutic agent for stress-related disorders.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_05:58:32.982.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiyi Xu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-22 20:41:15	ID requested
⏵ 1	2025-05-07 05:58:33	announced

Xu J, Ge Z, Wang H, Zhang C, Xu J, Li Y, Yang X, Zhang L, Li Z, Liu Z, Wang G, Du J, Long-term GABA supplementation mitigates anxiety by modulating complement and neuroinflammatory pathways. NPJ Sci Food, 9(1):60(2025) [pubmed]

10.1038/s41538-025-00423-w;

submitter keyword: ANXIETY,GABA

JING DU
contact affiliation	The National Clinical Research Center for Mental Disorders & Beijing Key Laboratory of Mental Disorders, Beijing Anding Hospital, Capital Medical University, Beijing, China
contact email	dujing2020@ccmu.edu.cn
lab head
Jiyi Xu
contact affiliation	Beijing Key Laboratory of Mental Disorders, National Clinical Research Center for Mental Disorders & National Center for Mental Disorders, Beijing Anding Hospital, Capital Medical University, Beijing, China
contact email	xujiyi22@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD059153
     1. Label: PRIDE project
     2. Name: Proteomic and Behavioral Study of the Anxiolytic Effects of GABA in a Chronic Restraint Stress-Induced Anxiety Model