PXD059147

PXD059147 is an original dataset announced via ProteomeXchange.

Title	Molecular characterization of perineural invasion-competent cancer cells using a novel in vitro model
Description	Invasion of cancer cells into peripheral nerves, known as perineural invasion (PNI), is an established risk factor for cancer recurrence and metastasis. However, there are no approaches available to specifically target PNI-competent cancer cells for diagnostic and therapeutic purposes. The lack of ideal preclinical models simulating natural PNI challenges the true characterization of PNI cells and understanding of the cellular events underlying PNI. In this work, we developed an in vitro model recapitulating the biological events associated with natural PNI. The novel in vitro model included intact nerve preparations in 3D culture format to facilitate natural PNI. Using the novel model, we found that autonomic neurosignaling promotes PNI demonstrating the utility of this model in exploring the fundamental biology of PNI. Using this model, we also demonstrated that PNI cells have survival advantages against cancer chemotherapy further demonstrating the utility of this model in screening potential anti-PNI therapies. We also characterized the molecular profile of PNI-competent breast cancer cells purified from the in vitro model. Follow-up analysis showed that CPLANE1 and hemopexin are potential markers of PNI cells for future theranostics purposes. Overall, this work presents a new in vitro model of PNI, which can be used to study the fundamentals of PNI and screen potential anti-PNI therapies.
HostingRepository	PRIDE
AnnounceDate	2025-07-14
AnnouncementXML	Submission_2025-07-13_16:07:30.211.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nickson Joseph
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	6520A Quadrupole Time-of-Flight LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-22 20:27:11	ID requested
⏵ 1	2025-07-13 16:07:31	announced

10.1038/s41420-025-02616-4;

Joseph N, Shrestha S, Sassmannshausen I, Mitzkus S, Chumala P, Sivakumar B, Baiju V, Ahmed S, Rees H, Katselis GS, Krishnan A, Defining the cellular and molecular features of nerve-invaded cancer cells using a newly characterized experimental model. Cell Death Discov, 11(1):314(2025) [pubmed]

submitter keyword: breast cancer, cancer recurrence, metastasis.,Perineural invasion, prostate cancer, cancer neuroscience

Anand Krishnan
contact affiliation	Dept. of Anatomy, Physiology, and Pharmacology College of Medicine University of Saskatchewan Saskatoon, Canada
contact email	anand.krishnan@usask.ca
lab head
Nickson Joseph
contact affiliation	Department of Anatomy Physiology Pharmacology, College of Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 5E5
contact email	hji826@usask.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD059147

PRIDE project URI

• PRIDE
  1. PXD059147
     1. Label: PRIDE project
     2. Name: Molecular characterization of perineural invasion-competent cancer cells using a novel in vitro model