PXD059132

PXD059132 is an original dataset announced via ProteomeXchange.

Title	A Metal-Dependant Formate Dehydrogenase Advances the Formate Bio-economy
Description	In this manuscript a multi-subunit metal dependent FDH is functionally expressed in a strain of E. coli engineered to assimilate all energy and biomass from formate. The replacement of the previously described FDH with this new, faster FDH allows for improved growth parameters rivaling natural formatotrophs for the first time. This new, robsulty growing formatotrophic E. coli was engineered to produce mevalonate and achieved the highest reported titer for any bioproduct from formate in fed batch mode. Finally the authors explored bioproducion from electrochemically-derived and lignin-derived formate, demonstrating the first example of a bioproduct produced using liginin-derived formate as a feedstock.
HostingRepository	PRIDE
AnnounceDate	2025-07-14
AnnouncementXML	Submission_2025-07-13_16:10:38.783.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christopher Petzold
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-22 14:50:45	ID requested
⏵ 1	2025-07-13 16:10:39	announced

Cowan AE, Hillers M, Rainaldi V, Collas F, Choudhary H, Zakaria BS, Bieberach GG, Carruthers DN, Grabovac M, Gin JW, Cawthon B, Chen Y, Turumtay EA, Baidoo EEK, Petzold CJ, Feist AM, Tejedor-Sanz S, Kensy F, Simmons BA, Keasling JD, Claassens NJ, Fast growth and high-titer bioproduction from renewable formate via metal-dependent formate dehydrogenase in Escherichia coli. Nat Commun, 16(1):5908(2025) [pubmed]

10.1038/s41467-025-61001-y;

submitter keyword: isoprenol, mevalonate, JBEI,Formate dehydrogenase, formate, reductive glycine pathway, bioproducion

Christopher J.
contact affiliation	Staff Scientist Biological Systems & Engineering Division Lawrence Berkeley National Laboratory Berkeley CA 94720
contact email	cjpetzold@lbl.gov
lab head
Christopher Petzold
contact affiliation	Lawrence Berkeley National Laboratory
contact email	cjpetzold@lbl.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD059132

PRIDE project URI

• PRIDE
  1. PXD059132
     1. Label: PRIDE project
     2. Name: A Metal-Dependant Formate Dehydrogenase Advances the Formate Bio-economy