PXD059131

PXD059131 is an original dataset announced via ProteomeXchange.

Title	HSP70 as a mediator of host-pathogen interaction in Arabidopsis thaliana during Plasmodiophora brassicae infection
Description	The interaction between Plasmodiophora brassicae and Arabidopsis thaliana reveals a complex struggle for cellular control, with HSP70 proteins playing a pivotal role. We demonstrate that Plasmodiophora infection induces HSP70 accumulation in Arabidopsis roots, and mutations in specific HSP70 isoforms either promote (HSP70-1, HSP70-13, HSP70-14) or suppress (HSP70-5, HSP70-12) the onset of clubroot disease. Analysis of mutant lines revealed that mutations in HSP70 trigger compensatory accumulation of other isoforms, underscoring the dynamic regulation of the chaperone network. Proteomic profiling of root galls during infection showed strong correlations between pathogen load and the abundance of Plasmodiophora HSP70 protein CEO96729. Interactomics analyses revealed that CEO96729 interacts with host proteins involved in Plasmodiophora responses, including extracellular GDSL esterase/lipase, and forms heterodimers with host HSP70 isoforms. These findings suggest that Plasmodiophora hijacks the host's chaperone machinery to facilitate its infection, offering a potential explanation for the observed modulation of disease progression in HSP70 mutants. Notably, the results also point to possible interactions with key enzymes in host physiology, including catalase 2, essential for ROS metabolism, and nitrilase, critical for auxin biosynthesis and root gall formation. These interactions highlight the potential involvement of CEO96729 in manipulating host cellular processes and raise the intriguing possibility of an unexpected role for this pathogen-derived HSP70 ortholog within the host cell. Collectively, our study highlights the multifaceted roles of HSP70 proteins in Plasmodiophora pathogenicity and host-pathogen interactions, offering insights into chaperone-mediated processes in plant immunity and infection dynamics.
HostingRepository	PRIDE
AnnounceDate	2025-05-21
AnnouncementXML	Submission_2025-05-21_06:05:34.679.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Cerny
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-22 14:16:35	ID requested
⏵ 1	2025-05-21 06:05:35	announced

10.1111/PPL.70309;

submitter keyword: Club root disease

Proteomics

Chaperone

Plant immunity

Interactomics

Plant-pathogen interaction

Martin Cerny
contact affiliation	Mendel University in Brno
contact email	martincerny83@gmail.com
lab head
Martin Cerny
contact affiliation	Mendel University in Brno
contact email	martincerny83@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD059131
     1. Label: PRIDE project
     2. Name: HSP70 as a mediator of host-pathogen interaction in Arabidopsis thaliana during Plasmodiophora brassicae infection