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PXD059118

PXD059118 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleClick-linking: a cell-compatible protein crosslinking method based on click chemistry
DescriptionWe developed a novel approach to in situ crosslinking mass spectrometry (XL-MS) where the reaction is split into two sequential and orthogonal coupling events. The method involves pre-stabilization of the proteome using a fixation protocol, followed by a two-step process that begins with extensive labeling of surface-available lysines with click-chemistry compatible reagents modified with NHS Esters. The second step involves the copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction of the installed precursors, which generates crosslinks with high efficiency. We observed approximately a 20-fold increase in protein-protein interactions detected by this approach when compared to standard DSS crosslinkers, and our Click-linking strategy even outperformed enrichable crosslinkers such as PhoX.
HostingRepositoryPRIDE
AnnounceDate2025-01-29
AnnouncementXMLSubmission_2025-01-28_22:17:43.652.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBruno Amaral
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Astral; Orbitrap Ascend
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-12-21 13:53:24ID requested
12025-01-28 22:17:44announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: in situ,Crosslinking, proteome-wide, protein-protein interactions, interactome
Contact List
David C.
contact affiliationDepartment of Chemstry & Department of Biochemistry and Molecular Biology, University of Clagary, Canada
contact emaildschriem@ucalgary.ca
lab head
Bruno Amaral
contact affiliationUniversity of Calgary
contact emailbruno.amaral@ucalgary.ca
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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