PXD059118 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Click-linking: a cell-compatible protein crosslinking method based on click chemistry |
Description | We developed a novel approach to in situ crosslinking mass spectrometry (XL-MS) where the reaction is split into two sequential and orthogonal coupling events. The method involves pre-stabilization of the proteome using a fixation protocol, followed by a two-step process that begins with extensive labeling of surface-available lysines with click-chemistry compatible reagents modified with NHS Esters. The second step involves the copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction of the installed precursors, which generates crosslinks with high efficiency. We observed approximately a 20-fold increase in protein-protein interactions detected by this approach when compared to standard DSS crosslinkers, and our Click-linking strategy even outperformed enrichable crosslinkers such as PhoX. |
HostingRepository | PRIDE |
AnnounceDate | 2025-01-29 |
AnnouncementXML | Submission_2025-01-28_22:17:43.652.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Bruno Amaral |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Astral; Orbitrap Ascend |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-12-21 13:53:24 | ID requested | |
⏵ 1 | 2025-01-28 22:17:44 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: in situ,Crosslinking, proteome-wide, protein-protein interactions, interactome |
Contact List
David C. |
contact affiliation | Department of Chemstry & Department of Biochemistry and Molecular Biology, University of Clagary, Canada |
contact email | dschriem@ucalgary.ca |
lab head | |
Bruno Amaral |
contact affiliation | University of Calgary |
contact email | bruno.amaral@ucalgary.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD059118
- Label: PRIDE project
- Name: Click-linking: a cell-compatible protein crosslinking method based on click chemistry