PXD059118

PXD059118 is an original dataset announced via ProteomeXchange.

Title	Click-linking: a cell-compatible protein crosslinking method based on click chemistry
Description	We developed a novel approach to in situ crosslinking mass spectrometry (XL-MS) where the reaction is split into two sequential and orthogonal coupling events. The method involves pre-stabilization of the proteome using a fixation protocol, followed by a two-step process that begins with extensive labeling of surface-available lysines with click-chemistry compatible reagents modified with NHS Esters. The second step involves the copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction of the installed precursors, which generates crosslinks with high efficiency. We observed approximately a 20-fold increase in protein-protein interactions detected by this approach when compared to standard DSS crosslinkers, and our Click-linking strategy even outperformed enrichable crosslinkers such as PhoX.
HostingRepository	PRIDE
AnnounceDate	2025-01-29
AnnouncementXML	Submission_2025-01-28_22:17:43.652.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bruno Amaral
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Astral; Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-21 13:53:24	ID requested
⏵ 1	2025-01-28 22:17:44	announced

Dataset with its publication pending

submitter keyword: in situ,Crosslinking, proteome-wide, protein-protein interactions, interactome

David C.
contact affiliation	Department of Chemstry & Department of Biochemistry and Molecular Biology, University of Clagary, Canada
contact email	dschriem@ucalgary.ca
lab head
Bruno Amaral
contact affiliation	University of Calgary
contact email	bruno.amaral@ucalgary.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD059118

PRIDE project URI

• PRIDE
  1. PXD059118
     1. Label: PRIDE project
     2. Name: Click-linking: a cell-compatible protein crosslinking method based on click chemistry