PXD059090

PXD059090 is an original dataset announced via ProteomeXchange.

Title	Microbial alginate foraging is conserved in geographically and taxonomically distinct ruminant microbiomes
Description	Saccharina latissima is a brown alga commonly found in the North Atlantic, Arctic and Pacific oceans. Alginate constitutes a substantial portion of the cell wall of S. latissima, and its digestion requires a specific set of enzymes, alginate lyases. We investigated if and how S. latissima is metabolized in geographically distinct rumen ecosystems through in vivo lamb feeding experiments (2.5 and 5% inclusion, DM basis) and in vitro cattle-based rumen simulation technique, RUSITEC, experiments (up to 50% inclusion). Evidence supporting ruminal degradation of alginate was explored using a combination of 16S rRNA sequencing, metagenomics and metaproteomics, physiology (fluorescently labelled S. latissima hot water extracts (FLA-SLAT)) and biochemical characterization of PL6 alginate lyases.
HostingRepository	PRIDE
AnnounceDate	2026-04-16
AnnouncementXML	Submission_2026-04-16_14:22:47.348.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Live Hagen
SpeciesList	scientific name: organismal metagenomes; NCBI TaxID: NEWT:410656;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-20 04:19:20	ID requested
⏵ 1	2026-04-16 14:22:47	announced

10.1038/S41467-026-72045-Z;

submitter keyword: None

Phillip Pope
contact affiliation	Norwegian University of Life Sciences
contact email	Phil.pope@nmbu.no
lab head
Live Hagen
contact affiliation	Faculty of Biotechnology, Chemistry and Food Science, Norwegian University of Life Sciences, Norway
contact email	live.hagen@nmbu.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD059090

PRIDE project URI

• PRIDE
  1. PXD059090
     1. Label: PRIDE project
     2. Name: Microbial alginate foraging is conserved in geographically and taxonomically distinct ruminant microbiomes