PXD059068 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Redox proteomics analysis of Arabidopsis wild type and grxs12 mutants |
| Description | For an overall evaluation of the protein redox status in grxs12 leaves, the iodoTMT-based redox proteomics approach was employed. After protein extraction, the free thiols in Cys were NEM-blocked, and then the Cys thiols with reversible oxidations such as sulfenic acids (-SOH), S-glutathionylation (-SSG), and disulfide bonds were reduced by DTT. Finally, the newly generated thiols in Cys were specifically labeled with iodoTMT-tags. The redox proteomics identified 227 peptides (162 proteins) exhibited changes in the redox status in grxs12 mutants compared to WT, including 88 more oxidized peptides and 139 less oxidized peptides. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-01-16 |
| AnnouncementXML | Submission_2026-01-15_20:10:17.449.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yanshuang Liu |
| SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702; |
| ModificationList | cysTMT6plex reporter+balance reagent cysteine disulfide; monohydroxylated residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-12-19 20:53:12 | ID requested | |
| ⏵ 1 | 2026-01-15 20:10:17 | announced | |
Publication List
Keyword List
| submitter keyword: Arabidopsis,Redox proteomics, grxs12 mutants, iodoTMT label |
Contact List
| Shaojun Dai |
|---|
| contact affiliation | Shanghai Normal University,Shanghai,China |
| contact email | daishaojun@shnu.edu.cn |
| lab head | |
| Yanshuang Liu |
|---|
| contact affiliation | Shanghai Normal University |
| contact email | 920662600@qq.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD059068
- Label: PRIDE project
- Name: Redox proteomics analysis of Arabidopsis wild type and grxs12 mutants
