PXD059066

PXD059066 is an original dataset announced via ProteomeXchange.

Title	Peritoneal Cell-Derived Exosomes: Novel Mediators of T Cell Activation and Vaccine Potency in Fish
Description	Exosomes are small extracellular vesicles that play significant roles in cell communication and immune modulation. When considering adaptive immunity, exosomes have been proposed to be involved in T and B cell development, antigen presentation, as well as the cross-presentation of antigens. While the potential of exosomes to carry vaccine-relevant antigens has been discussed, their intrinsic properties in activating adaptive immune responses have not yet been fully addressed. To explore these roles further, this study utilizes a fish model to examine the detailed effects of exosomes. We demonstrate that the peritoneal cells derived exosomes (PDEs), which fuse with cells in the peritoneum, carry and transport antigen presentation molecules locally. Additionally, PDEs are transferred from the peritoneum and accumulate in critical lymphoid organs, including the head kidney and spleen. Notably, PDEs effectively promoted Cd4-1+ and Cd4-2+ T cells activation in both in vivo and in vitro settings following exposure of cells to the PDEs. The experimental outcomes have indicated an increased level of Mhcii+ and Cd80/86+ exosomes secreted from peritoneal cells of fish vaccinated with Vibrio anguillarum via intraperitoneal injection, accompanied by the carriage of T cell-associated RNA and miRNA cargos. Moreover, the intraperitoneal injection of endogenous exosomes derived from Vibrio anguillarum-elicited cells enhanced vaccine potency by promoting antibody production. This study provides a novel avenue wherein exosomes are directly involved in vaccine-induced immunity, as evidenced in a lower vertebrate. It is plausible that such events and processes may also prevail in higher vertebrates, such as in humans.
HostingRepository	PRIDE
AnnounceDate	2026-04-06
AnnouncementXML	Submission_2026-04-05_16:19:02.750.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	xueyan shi
SpeciesList	scientific name: Paralichthys olivaceus; NCBI TaxID: NEWT:8255;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-19 20:00:27	ID requested
⏵ 1	2026-04-05 16:19:03	announced

10.1186/s12964-026-02752-z;

Shi X, Jiang L, Ye M, Yanglang A, Mikkelborg MK, Tang X, Xing J, Sheng X, Zhan W, Dalmo RA, Chi H, T cells and enhancement of vaccine efficacy in teleost. Cell Commun Signal, 24(1):(2026) [pubmed]

submitter keyword: vaccine potency, antibody response, T-cell activation

fish,Exosomes

Xueyan Shi
contact affiliation	Ocean University of China
contact email	shixueyan718@163.com
lab head
xueyan shi
contact affiliation	19506151061
contact email	shixueyan718@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD059066

PRIDE project URI

• PRIDE
  1. PXD059066
     1. Label: PRIDE project
     2. Name: Peritoneal Cell-Derived Exosomes: Novel Mediators of T Cell Activation and Vaccine Potency in Fish