PXD059054

PXD059054 is an original dataset announced via ProteomeXchange.

Title	Yeast TIA1 coordinates with Npl3 to promote ATG1 translation during starvation
Description	Macroautophagy/autophagy is crucial for maintaining metabolic homeostasis and cell survival during nutrient starvation. Autophagy requires the coordinated function of several Atg proteins, including the Atg1 kinase, for efficient induction and execution. Recently, several RNA-binding proteins have been shown to interact with the ATG1 transcript. However, a comprehensive understanding of the interaction network has yet to be elucidated. Here, we developed and utilized an approach to identify RNA-binding proteins that specifically interact with ATG1 untranslated regions. Our investigation revealed Npl3 and Pub1 as novel regulators of autophagy, and Atg1 protein translation by targeting its 5' UTR and 3' UTR, respectively. We show that Npl3 is required for the Pub1-ATG1 interaction and export to the cytoplasm. Subsequently, Pub1 interacts with the translational machinery and facilitates recruitment of polysomes to the ATG1 transcript, promoting its translation and thus autophagy induction. Significantly, in non-small cell lung cancer cell lines, TIA1 upregulates ULK1 protein expression at the post-transcriptional level, thereby positively regulating autophagy. Overall, our study highlights the intricate regulatory landscape that fine-tunes ATG1 mRNA export and translation, thereby uncovering several novel regulators of the autophagy process.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_06:03:56.926.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shree Padma Metur
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-19 11:13:29	ID requested
⏵ 1	2025-05-07 06:03:57	announced

Metur SP, Song X, Mehta S, Dialynaki D, Bhattacharyya D, Yin Z, Tang D, Klionsky DJ, Yeast TIA1 coordinates with Npl3 to promote ATG1 translation during starvation. Cell Rep, 44(2):115316(2025) [pubmed]

10.1016/j.celrep.2025.115316;

submitter keyword: mRNA metabolism, RBPs,Autophagy, post-transcriptional regulation, translation

Daniel Klionsky
contact affiliation	University of Michigan
contact email	klionsky@umich.edu
lab head
Shree Padma Metur
contact affiliation	University of Michigan
contact email	spadma@umich.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD059054
     1. Label: PRIDE project
     2. Name: Yeast TIA1 coordinates with Npl3 to promote ATG1 translation during starvation