⮝ Full datasets listing
PXD059042
PXD059042 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Membrane damage induces TagQ/TagR complex-mediated H1-T6SS assembly in Pseudomonas aeruginosa |
| Description | The type VI secretion system (T6SS) is a large macromolecular complex crucial for inter-bacterial competition. Its expression and localization are tightly regulated, with the anti-bacterial H1-T6SS in Pseudomonas aeruginosa assembling in response to membrane damage. While several proteins are known to regulate this response, the mechanism remains unknown. Here, we used biochemical approaches to show formation of a complex between the membrane lipoprotein TagQ and the periplasmic protein TagR. Furthermore, Co-IP and in silico modeling indicates TagQ/R complex interactions with PpkA, likely dimerizing the kinase to initiate a phosphorylation cascade leading to H1-T6SS assembly. Mass spectrometry analysis of phosphorylated proteins in P. aeruginosa showed that PpkA does not have any targets outside of the previously reported Fha1, suggesting the sensor module acts exclusively to regulate T6SS assembly. Our work reveals the functional basis of the TagQR-PpkA signaling pathway and links membrane damage to localized H1-T6SS assembly, which is critical for efficient retaliation during inter-bacterial interactions. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-04-02 |
| AnnouncementXML | Submission_2026-04-02_02:53:28.198.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Thomas Bock |
| SpeciesList | scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964; |
| ModificationList | No PTMs are included in the dataset; Phospho |
| Instrument | Orbitrap Exploris 480; Orbitrap Fusion Lumos; Q Exactive Plus; Q Exactive HF; Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-12-19 03:02:53 | ID requested | |
| ⏵ 1 | 2026-04-02 02:53:28 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: T6SS, TagQ/TagR, DatasetType:Proteomics |
Contact List
| Alexander Schmidt | |
|---|---|
| contact affiliation | Biozentrum, Universtiy of Basel, 4056 Basel, Switzerland |
| contact email | alex.schmidt@unibas.ch |
| lab head | |
| Thomas Bock | |
| contact affiliation | Uni Basel |
| contact email | thomas.bock@unibas.ch |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000096700/ |
