PXD059026

PXD059026 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	CPT1A-mediated MFF succinylation promotes stemness maintenance in ovarian cancer stem cells
Description	Cancer stem cells (CSCs) play crucial roles in cancer progression, immune evasion, drug resistance, and recurrence. Understanding the mechanisms behind CSCs generation and stemness maintenance is vital for early cancer diagnosis and treatment. Here, we unveil that carnitine palmitoyltransferase 1A (CPT1A) is highly expressed in ovarian cancer stem cells (OCSCs) and is essential for maintaining stemness by regulating lipid desaturation. Studies confirmed that CPT1A enhances SREBP1 activation, upregulating SCD1 expression, and promoting lipid desaturation in OCSCs. Mechanistic studies reveal that CPT1A promotes succinylation of mitochondrial fission factor (MFF) through its lysine succinyltransferase (LSTase) activity, crucial for mitochondria-associated membranes formation and SREBP1 activation. Inhibiting CPT1A's LSTase activity with Glyburide reduces OCSCs' stemness and enhances cisplatin's anti-tumor effects against ovarian cancer in vitro and in vivo. Together, our studies highlight the significance of CPT1A's LSTase activity in maintaining OCSCs' stemness, offering potential targets and therapeutic strategies for ovarian cancer treatment.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_06:00:54.545.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yaqin Zhu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-18 20:31:11	ID requested
⏵ 1	2025-05-07 06:00:55	announced

Publication List

10.1038/s42003-025-07720-w;
Zhu Y, Chen S, Su H, Meng Y, Zang C, Ning P, Hu L, Shao H, CPT1A-mediated MFF succinylation promotes stemness maintenance in ovarian cancer stem cells. Commun Biol, 8(1):250(2025) [pubmed]

10.1038/s42003-025-07720-w;

Zhu Y, Chen S, Su H, Meng Y, Zang C, Ning P, Hu L, Shao H, CPT1A-mediated MFF succinylation promotes stemness maintenance in ovarian cancer stem cells. Commun Biol, 8(1):250(2025) [pubmed]

Keyword List

submitter keyword: MFF, stemness maintenance, CPT1A,ovarian cancer stem cells, MAMs

submitter keyword: MFF, stemness maintenance, CPT1A,ovarian cancer stem cells, MAMs

Contact List

Huanjie Shao
contact affiliation	Shaanxi normal university
contact email	hshao@snnu.edu.cn
lab head
Yaqin Zhu
contact affiliation	Shaanxi Normal University
contact email	15536387032@163.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD059026
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD059026

PRIDE project URI

Repository Record List

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