PXD059009 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Protein modifications by advanced glycation end products (AGEs) in human clear cell renal cell carcinoma |
| Description | The development of clear cell renal cell carcinoma (ccRCC) is connected with disturbed glycolytic processes leading to the highly reactive dicarbonyls generation, resulting in protein modifications by advanced glycation end products (AGEs). Therefore, the present study aimed to analyze the level of major AGE-protein adducts and identify modified proteins using a proteomic approach in ccRCC tissue of cancer patients compared to nonchanged tissue. The obtained results indicated a higher level of carboxymethylation/carboxyethylation and increased formation of pyrraline, argpyrimidine and pentosidine on cysteine, lysine or arginine residues in tumor tissue comparing to non-tumor kidney tissue. The main protein creating adducts with AGEs was albumin in both forms, pre-proalbumin and mature protein. The majority of the AGE-modified proteins in ccRCC tissues were proteins with catalytic and binding activity, regulators, transporters, and transcription activity, localized in cellular components, including nucleus what was confirmed by immunofluorescence assay. Within ten glycolytic enzymes modified by AGEs, five were indicated only in ccRCC tissue. Summarizing, obtained results clearly indicates the role of AGEs and their adducts formation with proteins in the development of kidney tumor. Moreover, it can be concluded that there is a real possibility of using knowledge regarding the consequences of the formation of AGE-protein connections in the assessment of the therapy effectiveness. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-09 |
| AnnouncementXML | Submission_2026-02-08_16:23:39.548.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Agnieszka Gęgotek |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-12-18 12:33:52 | ID requested | |
| ⏵ 1 | 2026-02-08 16:23:40 | announced | |
Publication List
| 10.1186/s12967-025-06958-6; |
| G, ę, gotek A, Bra, ń, ska-Januszewska J, Samocik P, Koz, ł, owski R, Koda M, Zarkovic N, Skrzydlewska E, Ostrowska H, Protein modifications by advanced glycation end products (AGEs) in human clear cell renal cell carcinoma. J Transl Med, 23(1):914(2025) [pubmed] |
Keyword List
| submitter keyword: advanced glycation end products (AGEs) |
| protein modification |
| proteomics |
| kidney disease |
| tumor |
| clear cell renal cell carcinoma (ccRCC); |
Contact List
| Agnieszka Gęgotek |
|---|
| contact affiliation | UMB |
| contact email | agnieszka.gegotek@umb.edu.pl |
| lab head | |
| Agnieszka Gęgotek |
|---|
| contact affiliation | Medical University of Białystok |
| contact email | agnieszkagegotek@o2.pl |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD059009 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD059009
- Label: PRIDE project
- Name: Protein modifications by advanced glycation end products (AGEs) in human clear cell renal cell carcinoma
