PXD058986

PXD058986 is an original dataset announced via ProteomeXchange.

Title	LYM2 mediates chitin-induced plasmodesmal flux reduction in Populus x canescens
Description	LYSIN MOTIF DOMAIN-CONTAINING GLYCOSYLPHOSPHATIDYL INOSITOL-ANCHORED PROTEIN 2 (LYM2) is a GPI-anchored LysM receptor-like protein (LysM-RLP) that harbors three extracellular LysM-domains and a GPI moiety at the C-terminus. LYM2 in Arabidopsis thaliana is involved in chitin-mediated plasmodesmal flux regulation. The poplar paralogs PcLYM2-1 and PcLYM2-2 were identified and characterized in this study. PcLYM2-2 exhibits tissue-specific alternative splicing resulting in variants that differ exclusively at the first exon encoding the three LysM domains. Chitin-binding assays showed that all identified PcLYM2 proteins bind chitin, but with different capacity. Subcellular localization studies in Nicotiana benthamiana indicated that all identified PcLYM2 proteins localize at plasmodesmata, suggesting a role of these proteins in plasmodesmata-related functions. Plasmodesmal flux analysis of wildtype poplar and Pclym2-1 Pclym2-2 double knockout lines showed that PcLYM2 proteins mediate chitin-triggered PD closure. Loss-of-function of PcLYM2-1 is sufficient to abolish chitin-induced PD closure, suggesting that PcLYM2-1 is the primary LysM-RLP in poplar involved in this process.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-06_16:13:56.365.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oliver Valerius
SpeciesList	scientific name: Populus canescens (Grey poplar) (Populus tremula x Populus alba); NCBI TaxID: NEWT:80863;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-18 06:06:13	ID requested
⏵ 1	2026-09-06 16:13:57	announced

10.3389/fpls.2026.1879338;

Awwanah M, Reinhold M, Niemann G, Muhr M, Schmitt K, Valerius O, Majcherczyk A, Janz D, Petutschnig E, Lipka V, Teichmann T, . Front Plant Sci, 17():1879338(2026) [pubmed]

submitter keyword: plasmodesmal flux,LysM-RLP, alternative splicing, chitin-binding affinity

Dr. Thomas Teichmann
contact affiliation	Georg-August-University Göttingen, Schwann-Schleiden Research Center for Molecular Cell Biology, Department of Plant Cell Biology, Germany
contact email	tteichm@gwdg.de
lab head
Oliver Valerius
contact affiliation	Georg-August-Universität Göttingen
contact email	ovaleri@gwdg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058986
     1. Label: PRIDE project
     2. Name: LYM2 mediates chitin-induced plasmodesmal flux reduction in Populus x canescens