PXD058985 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The related EIF4G3 and EIF4G4 initiation factors from Leishmania: dissimilar modes of action during translation revealed by a comparative proteomic approach |
| Description | mRNA translation in eukaryotes starts with the binding of the eIF4F complex to its capped 5' end. eIF4F is formed by the joining of its eIF4G and eIF4E subunits, with the former mediating relevant interactions required for translation to proceed. Five eIF4G homologues are found in Leishmania, as part of multiple eIF4F-like complexes, all likely active in translation. Here we performed a large-scale comparative analysis of protein partners associated with two of these eIF4Gs, the related EIF4G3 and EIF4G4. Different transgenic Leishmania infantum cell lines were generated expressing these proteins tagged with a C-terminal HA epitope, each capable of complementing the deletion of the native protein. Proteins associated with the HA-tagged eIF4Gs, after immunoprecipitation with anti-HA beads, were identified through mass spectrometry from three different growth phases: early exponential (24hs), late exponential (48hs) and stationary (96hs). EIF4G3 and EIF4G4 co-precipitated with known and novel protein partners belonging to different functional categories, with some restricted to selected growth phases. The results are thus compatible with the two eIF4Gs likely having related but distinct functions during translation and possibly being targeted by different regulatory mechanisms which are affected by cell growth. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-13 |
| AnnouncementXML | Submission_2026-04-12_16:23:36.206.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Stéphanny Oliveira |
| SpeciesList | scientific name: Leishmania infantum JPCM5; NCBI TaxID: NEWT:435258; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | LTQ Orbitrap XL ETD |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-12-18 05:55:37 | ID requested | |
| ⏵ 1 | 2026-04-12 16:23:37 | announced | |
Publication List
| 10.1186/s13071-026-07297-1; |
| Oliveira SSS, Melo do Nascimento L, Moura DMN, de Souza Reis CR, de Melo Neto OP, The related EIF4G3 and EIF4G4 initiation factors from Leishmania: dissimilar modes of action during translation revealed by a comparative proteomic approach. Parasit Vectors, 19(1):(2026) [pubmed] |
Keyword List
| submitter keyword: protein synthesis, gene expression regulation, trypanosomatid |
Contact List
| Osvaldo Pompílio de Melo Neto |
|---|
| contact affiliation | Microbiology departament, Aggeu Magalhães Institute, Brazil. |
| contact email | osvaldo.pompilio@fiocruz.br |
| lab head | |
| Stéphanny Oliveira |
|---|
| contact affiliation | Instituo Aggeu Magalhães |
| contact email | stephannys@fiocruz.br |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD058985
- Label: PRIDE project
- Name: The related EIF4G3 and EIF4G4 initiation factors from Leishmania: dissimilar modes of action during translation revealed by a comparative proteomic approach
