PXD058977

PXD058977 is an original dataset announced via ProteomeXchange.

Title	The secretome of the white-rot basidiomycete Abortiporus biennis LGAM 436 can cause significant modifications to polystyrene structure
Description	This study focused on investigating the biocatalytic potential of the white-rot basidiomycete Abortiporus biennis LGAM 436 to modify the structure of different types of PS, including amorphous film with atactic stereochemistry and commercial EPS foam. The selection of this specific strain was mainly based on a previous report by our group, focusing on the isolation of a laccase secreted by A. biennis LGAM 436, which is capable of reducing the number-average molecular weight of PS by 20% (10.1016/j.chemosphere.2022.137338). To expedite the process of whole cell biocatalysis, we also evaluated the addition of the phenol-rich olive-oil mill wastewater effluent (OOMW); a byproduct which can act as an inducer of oxidative enzymes such as laccases and LiPs (10.1016/j.jenvman.2016.02.042). Following the assessment of PS degradation through analyses of molar mass, the emergence of new functional groups, and alterations in surface morphology, we sought to elucidate the enzymatic activities expressed in the presence of PS. This was achieved after the secretome assessment of the basidiomycete via proteomics studies, which will pave the way for the heterologous expression of novel enzymatic activities that can synergistically act to modify and degrade polyolefin structures.
HostingRepository	PRIDE
AnnounceDate	2025-04-24
AnnouncementXML	Submission_2025-04-24_01:58:55.056.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD058977
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Romanos Siaperas
SpeciesList	scientific name: Abortiporus biennis; NCBI TaxID: 137743;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-18 02:38:24	ID requested
⏵ 1	2025-04-24 01:58:56	announced

10.1016/j.envpol.2025.126214;

Taxeidis G, Siaperas R, Foka K, Ponjavic M, Nikodinovic-Runic J, Zerva A, Topakas E, Elucidating the enzymatic response of the white rot basidiomycete Abortiporus biennis for the downgrade of polystyrene. Environ Pollut, 374():126214(2025) [pubmed]

10.6019/PXD058977;

ProteomeXchange project tag: EPIC-XS

submitter keyword: polystyrene, LFQ,plastic pollution, biodegradation, Abortiporus, fungi, white-rot, secretome, quantms, DDA

Evangelos Topakas
contact affiliation	Industrial Biotechnology & Biocatalysis Group, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, Athens, Greece
contact email	vtopakas@chemeng.ntua.gr
lab head
Romanos Siaperas
contact affiliation	Industrial Biotechnology & Biocatalysis Group, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, Athens, Greece
contact email	roman_siap@hotmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD058977

PRIDE project URI

• PRIDE
  1. PXD058977
     1. Label: PRIDE project
     2. Name: The secretome of the white-rot basidiomycete Abortiporus biennis LGAM 436 can cause significant modifications to polystyrene structure